TY - JOUR
T1 - Bioluminescent whole cell optical fiber sensor to genotoxicants
T2 - 5th European Conference on Optical Chemical Sensors and Biosensors
AU - Polyak, Boris
AU - Bassis, Efim
AU - Novodvorets, Alex
AU - Belkin, Shimshon
AU - Marks, Robert S.
PY - 2001/4/15
Y1 - 2001/4/15
N2 - An Escherichia coli strain, genetically modified to emit a luminescent signal in the presence of genotoxic agents, was alginate-immobilized onto an exposed core of a fiber-optic. The performance of this whole-cell optical fiber sensor system was examined as a function of several parameters, including gel probe matrix volume, bacterial cell density, numerical aperture of the fiber core and working temperature. An optimal response to a model genotoxicant, mitomycin C, was achieved with six alginate/bacterial adlayers on a 1 cm exposed fiber-optic core. Total alginate volume per tip was about 100 μl, containing a bacterial suspension of around 1.5-3.0 × 107 cells. When the core diameter was etched down to 270 μm, photon detection efficiency significantly increased, although to a lesser extent than that expected from theoretical calculations. Further reduction in core diameter led to a reduced performance. Activity at 37°C was superior to that at 26°C. Under these optimized conditions, optrode response was mitomycin C dose-dependent for at least 6 h, with a lower detection threshold of 25 μg/l.
AB - An Escherichia coli strain, genetically modified to emit a luminescent signal in the presence of genotoxic agents, was alginate-immobilized onto an exposed core of a fiber-optic. The performance of this whole-cell optical fiber sensor system was examined as a function of several parameters, including gel probe matrix volume, bacterial cell density, numerical aperture of the fiber core and working temperature. An optimal response to a model genotoxicant, mitomycin C, was achieved with six alginate/bacterial adlayers on a 1 cm exposed fiber-optic core. Total alginate volume per tip was about 100 μl, containing a bacterial suspension of around 1.5-3.0 × 107 cells. When the core diameter was etched down to 270 μm, photon detection efficiency significantly increased, although to a lesser extent than that expected from theoretical calculations. Further reduction in core diameter led to a reduced performance. Activity at 37°C was superior to that at 26°C. Under these optimized conditions, optrode response was mitomycin C dose-dependent for at least 6 h, with a lower detection threshold of 25 μg/l.
KW - Bioluminescent bacteria
KW - Genotoxicity
KW - Optical fiber biosensors
KW - Sodium alginate
UR - http://www.scopus.com/inward/record.url?scp=0035871854&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(00)00707-3
DO - 10.1016/S0925-4005(00)00707-3
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AN - SCOPUS:0035871854
SN - 0925-4005
VL - 74
SP - 18
EP - 26
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1-3
Y2 - 16 April 2000 through 19 April 2000
ER -