TY - JOUR
T1 - Biphasic influence of hypoxia on tuftelin expression in mouse mesenchymal C3H10T1/2 stem cells
AU - Deutsch, Dan
AU - Silverstein, Nechama
AU - Shilo, Dekel
AU - Lecht, Shimon
AU - Lazarovici, Philip
AU - Blumenfeld, Anat
PY - 2011/12
Y1 - 2011/12
N2 - Tuftelin, an acidic protein, thought to play a role in the initial stages of ectodermal enamel mineralization, has since been detected in mesenchymal-derived tissues. During bone/cartilage development and regeneration, mesenchymal stem cells (MSCs) undergo an avascular period in a hypoxic environment, involving induction of hypoxia-inducible factor 1-alpha (HIF-1-alpha), a key component in this process. In the present study we investigated, in a mouse mesenchymal C3H10T1/2 stem cell model, the hypothesis that oxygen stress modulates tuftelin 1 expression in relation to HIF-1-alpha (Hif1a), in a mouse mesenchymal C3H10T1/2 stem cell model. The results of the present study showed a biphasic induction of tuftelin, similar to the pattern of HIF-1-alpha expression, in MSCs subjected to a hypoxic insult of 1% O 2 through a period of 2-24h. Immunocytochemistry analysis of the cells exposed to hypoxic insult for 2-24h revealed the same biphasic pattern of tuftelin protein expression. Tuftelin localization appears to be mainly in the cytoplasm, and concentrated at the perinuclear region of the cells by 24h of hypoxic insult. Based on our previous studies using the neuronal PC12 cell model, in which tuftelin induction was mediated by Hif1a, we propose that tuftelin is a member of oxygen-sensitive genes and implicated in the adaptive mechanisms regulating MSC function.
AB - Tuftelin, an acidic protein, thought to play a role in the initial stages of ectodermal enamel mineralization, has since been detected in mesenchymal-derived tissues. During bone/cartilage development and regeneration, mesenchymal stem cells (MSCs) undergo an avascular period in a hypoxic environment, involving induction of hypoxia-inducible factor 1-alpha (HIF-1-alpha), a key component in this process. In the present study we investigated, in a mouse mesenchymal C3H10T1/2 stem cell model, the hypothesis that oxygen stress modulates tuftelin 1 expression in relation to HIF-1-alpha (Hif1a), in a mouse mesenchymal C3H10T1/2 stem cell model. The results of the present study showed a biphasic induction of tuftelin, similar to the pattern of HIF-1-alpha expression, in MSCs subjected to a hypoxic insult of 1% O 2 through a period of 2-24h. Immunocytochemistry analysis of the cells exposed to hypoxic insult for 2-24h revealed the same biphasic pattern of tuftelin protein expression. Tuftelin localization appears to be mainly in the cytoplasm, and concentrated at the perinuclear region of the cells by 24h of hypoxic insult. Based on our previous studies using the neuronal PC12 cell model, in which tuftelin induction was mediated by Hif1a, we propose that tuftelin is a member of oxygen-sensitive genes and implicated in the adaptive mechanisms regulating MSC function.
KW - Expression
KW - Hypoxia
KW - Hypoxia-inducible factor 1-alpha (HIF-1-alpha)
KW - Mesenchymal stem cells (MSCs)
KW - Tuftelin
UR - http://www.scopus.com/inward/record.url?scp=84855872752&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0722.2011.00861.x
DO - 10.1111/j.1600-0722.2011.00861.x
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C2 - 22243227
AN - SCOPUS:84855872752
SN - 0909-8836
VL - 119
SP - 55
EP - 61
JO - European Journal of Oral Sciences
JF - European Journal of Oral Sciences
IS - SUPPL.1
ER -