Biphasic influence of hypoxia on tuftelin expression in mouse mesenchymal C3H10T1/2 stem cells

Dan Deutsch*, Nechama Silverstein, Dekel Shilo, Shimon Lecht, Philip Lazarovici, Anat Blumenfeld

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Tuftelin, an acidic protein, thought to play a role in the initial stages of ectodermal enamel mineralization, has since been detected in mesenchymal-derived tissues. During bone/cartilage development and regeneration, mesenchymal stem cells (MSCs) undergo an avascular period in a hypoxic environment, involving induction of hypoxia-inducible factor 1-alpha (HIF-1-alpha), a key component in this process. In the present study we investigated, in a mouse mesenchymal C3H10T1/2 stem cell model, the hypothesis that oxygen stress modulates tuftelin 1 expression in relation to HIF-1-alpha (Hif1a), in a mouse mesenchymal C3H10T1/2 stem cell model. The results of the present study showed a biphasic induction of tuftelin, similar to the pattern of HIF-1-alpha expression, in MSCs subjected to a hypoxic insult of 1% O 2 through a period of 2-24h. Immunocytochemistry analysis of the cells exposed to hypoxic insult for 2-24h revealed the same biphasic pattern of tuftelin protein expression. Tuftelin localization appears to be mainly in the cytoplasm, and concentrated at the perinuclear region of the cells by 24h of hypoxic insult. Based on our previous studies using the neuronal PC12 cell model, in which tuftelin induction was mediated by Hif1a, we propose that tuftelin is a member of oxygen-sensitive genes and implicated in the adaptive mechanisms regulating MSC function.

Original languageEnglish
Pages (from-to)55-61
Number of pages7
JournalEuropean Journal of Oral Sciences
Volume119
Issue numberSUPPL.1
DOIs
StatePublished - Dec 2011

Keywords

  • Expression
  • Hypoxia
  • Hypoxia-inducible factor 1-alpha (HIF-1-alpha)
  • Mesenchymal stem cells (MSCs)
  • Tuftelin

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