Block of quantal end-plate currents of mouse muscle by physostigmine and procaine

J. Dudel*, M. Schramm, C. Franke, E. Ratner, H. Parnas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Quantal endplate currents (qEPCs) were recorded from hemidiaphragms of mice by means of a macro-patch-clamp electrode. Excitation was blocked with tetrodotoxin, and quantal release was elicited by depolarizing pulses through the electrode. Physostigmine (Phys) or procaine (Proc) was applied to the recording site by perfusion of the electrode tip. Low concentrations of Phys increased the amplitude and prolonged the decay time constants of qEPCs from ~3 to ~10 ms, due to block of acetylcholine-esterase. With 20 μM to 2 mM Phys or Proc, the decay of qEPCs became biphasic, an initial short time constant τ(s) decreasing to <1 ms with 1 mM Phys and to ~0.3 ms with 1 mM Proc. The long second time constant of the decay, τ1, reached values of ≤100 ms with these blocker concentrations. The blocking effects of Phys and Proc on the qEPC are due to binding to the open channel conformation. A method is described to extract the rate constants of binding (b(p)) from the sums 1/τ(s) + 1/τ1, and the rates of unbinding (b(-p)) from τ0 · τ(s)-1 · τ1-1 (τ(o) is the decay time constant of the control EPC). For Phys and Proc b(p) of 1.3 and 5 · 106 M-1 s-1 and b(-p) of 176 and 350 s-1, respectively, were found. Using these rate constants and a reaction scheme for the nicotinic receptor together with the respective rate constants determined before, we could model the experimental results satisfactorily.

Original languageEnglish
Pages (from-to)2386-2397
Number of pages12
JournalJournal of Neurophysiology
Volume81
Issue number5
DOIs
StatePublished - 1999

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