Abstract
Glycobiology is emerging as a primary field of interest for biological research. As such, it is crucial to develop and improve tools for the study of sugar-modified biomolecules. Many research endeavors in the field of protein glycobiology involve boiling of proteins in sample buffer for 5-10 minutes prior to gel
electrophoresis and their further characterization. Here we examined the effect of boiling purified glycoproteins, as well as a mixture of protein lysate, in sample buffer on their sugar content and found that prolonged boiling partially reduces detection of certain glycans, such as of the cytoplasmic OGlcNAc. This should be taken into consideration when characterizing glycans using common assays.
electrophoresis and their further characterization. Here we examined the effect of boiling purified glycoproteins, as well as a mixture of protein lysate, in sample buffer on their sugar content and found that prolonged boiling partially reduces detection of certain glycans, such as of the cytoplasmic OGlcNAc. This should be taken into consideration when characterizing glycans using common assays.
| Original language | American English |
|---|---|
| Pages (from-to) | 4-8 |
| Number of pages | 5 |
| Journal | CIBTech Journal of Bio-Protocols |
| Volume | 5 |
| Issue number | 1 |
| State | Published - 2016 |
Keywords
- Boiling
- Glycoproteins
- N-Linked Glycans
- O-Linked Glycans
- Sample Buffer