Bone regeneration with bovine bone impairs orthodontic tooth movement despite proper osseous wound healing in a novel mouse model

Yehuda Klein; Omer Fleissig; Ayala Stabholz; Stella Chaushu; David Polak

doi:10.1002/JPER.17-0550

Bone regeneration with bovine bone impairs orthodontic tooth movement despite proper osseous wound healing in a novel mouse model

Yehuda Klein, Omer Fleissig, Ayala Stabholz, Stella Chaushu, David Polak^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Background: The aim of this study was to investigate the biological mechanisms underlying alveolar bone regeneration (ABR) and orthodontic tooth movement into bovine bone (BB) regenerated sites. Methods: Two mouse models were established in C57BL/6 mice. The ABR model was based on osseous defects filled with BB. The orthodontic tooth movement-ABR model was used to move a molar into the regenerated site. Osseous morphometric analysis and tooth movement distance were evaluated with micro-CT. Histologic characteristics and osteoclast (OCS) accumulation were evaluated by hematoxylin and eosin and tartrate-resistant acid phosphatase staining (TRAP). Expression and location of the receptor activator of nuclear factor-kappa B (RANKL) and of osteoprotegerin (OPG) were evaluated by immunofluorescent staining. Results: Bone healing peaked at 4 weeks. The distance of the orthodontic tooth movement into the bovine bone was significantly reduced versus that of the nonbovine bone controls. BB particles accumulated along the root's pressure side during orthodontic treatment. Despite the osteoclasts’ presence adjacent to the BB particles, no BB resorption was observed. Increased RANKL expression was seen at the orthodontic tooth movement pressure zone, without any change in OPG expression. Conclusion: The two novel mouse models show that the lack of resorption of BB xenografts renders them inadequate for proper orthodontic tooth movement at a later stage.

Original language	American English
Pages (from-to)	189-199
Number of pages	11
Journal	Journal of Periodontology
Volume	90
Issue number	2
DOIs	https://doi.org/10.1002/JPER.17-0550
State	Published - 1 Feb 2019

Bibliographical note

Funding Information:
The study was funded by the Cabakoff Foundation, Hebrew University, Jerusalem, Israel. All authors have contributed to, seen, and approved the final submitted version of the manuscript. SC and DP contributed equally to this work. We hereby declare that this is an original manuscript, has not been sent elsewhere, that we do not have commercial or other associations that might pose a conflict of interest and that the study has been conducted according to the highest principals of animal welfare.

Publisher Copyright:
© 2018 American Academy of Periodontology

Keywords

bone regeneration
orthodontics
wound healing

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10.1002/JPER.17-0550

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title = "Bone regeneration with bovine bone impairs orthodontic tooth movement despite proper osseous wound healing in a novel mouse model",

abstract = "Background: The aim of this study was to investigate the biological mechanisms underlying alveolar bone regeneration (ABR) and orthodontic tooth movement into bovine bone (BB) regenerated sites. Methods: Two mouse models were established in C57BL/6 mice. The ABR model was based on osseous defects filled with BB. The orthodontic tooth movement-ABR model was used to move a molar into the regenerated site. Osseous morphometric analysis and tooth movement distance were evaluated with micro-CT. Histologic characteristics and osteoclast (OCS) accumulation were evaluated by hematoxylin and eosin and tartrate-resistant acid phosphatase staining (TRAP). Expression and location of the receptor activator of nuclear factor-kappa B (RANKL) and of osteoprotegerin (OPG) were evaluated by immunofluorescent staining. Results: Bone healing peaked at 4 weeks. The distance of the orthodontic tooth movement into the bovine bone was significantly reduced versus that of the nonbovine bone controls. BB particles accumulated along the root's pressure side during orthodontic treatment. Despite the osteoclasts{\textquoteright} presence adjacent to the BB particles, no BB resorption was observed. Increased RANKL expression was seen at the orthodontic tooth movement pressure zone, without any change in OPG expression. Conclusion: The two novel mouse models show that the lack of resorption of BB xenografts renders them inadequate for proper orthodontic tooth movement at a later stage.",

keywords = "bone regeneration, orthodontics, wound healing",

author = "Yehuda Klein and Omer Fleissig and Ayala Stabholz and Stella Chaushu and David Polak",

note = "Funding Information: The study was funded by the Cabakoff Foundation, Hebrew University, Jerusalem, Israel. All authors have contributed to, seen, and approved the final submitted version of the manuscript. SC and DP contributed equally to this work. We hereby declare that this is an original manuscript, has not been sent elsewhere, that we do not have commercial or other associations that might pose a conflict of interest and that the study has been conducted according to the highest principals of animal welfare. Publisher Copyright: {\textcopyright} 2018 American Academy of Periodontology",

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AU - Klein, Yehuda

AU - Fleissig, Omer

AU - Stabholz, Ayala

AU - Chaushu, Stella

AU - Polak, David

N1 - Funding Information: The study was funded by the Cabakoff Foundation, Hebrew University, Jerusalem, Israel. All authors have contributed to, seen, and approved the final submitted version of the manuscript. SC and DP contributed equally to this work. We hereby declare that this is an original manuscript, has not been sent elsewhere, that we do not have commercial or other associations that might pose a conflict of interest and that the study has been conducted according to the highest principals of animal welfare. Publisher Copyright: © 2018 American Academy of Periodontology

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Background: The aim of this study was to investigate the biological mechanisms underlying alveolar bone regeneration (ABR) and orthodontic tooth movement into bovine bone (BB) regenerated sites. Methods: Two mouse models were established in C57BL/6 mice. The ABR model was based on osseous defects filled with BB. The orthodontic tooth movement-ABR model was used to move a molar into the regenerated site. Osseous morphometric analysis and tooth movement distance were evaluated with micro-CT. Histologic characteristics and osteoclast (OCS) accumulation were evaluated by hematoxylin and eosin and tartrate-resistant acid phosphatase staining (TRAP). Expression and location of the receptor activator of nuclear factor-kappa B (RANKL) and of osteoprotegerin (OPG) were evaluated by immunofluorescent staining. Results: Bone healing peaked at 4 weeks. The distance of the orthodontic tooth movement into the bovine bone was significantly reduced versus that of the nonbovine bone controls. BB particles accumulated along the root's pressure side during orthodontic treatment. Despite the osteoclasts’ presence adjacent to the BB particles, no BB resorption was observed. Increased RANKL expression was seen at the orthodontic tooth movement pressure zone, without any change in OPG expression. Conclusion: The two novel mouse models show that the lack of resorption of BB xenografts renders them inadequate for proper orthodontic tooth movement at a later stage.

AB - Background: The aim of this study was to investigate the biological mechanisms underlying alveolar bone regeneration (ABR) and orthodontic tooth movement into bovine bone (BB) regenerated sites. Methods: Two mouse models were established in C57BL/6 mice. The ABR model was based on osseous defects filled with BB. The orthodontic tooth movement-ABR model was used to move a molar into the regenerated site. Osseous morphometric analysis and tooth movement distance were evaluated with micro-CT. Histologic characteristics and osteoclast (OCS) accumulation were evaluated by hematoxylin and eosin and tartrate-resistant acid phosphatase staining (TRAP). Expression and location of the receptor activator of nuclear factor-kappa B (RANKL) and of osteoprotegerin (OPG) were evaluated by immunofluorescent staining. Results: Bone healing peaked at 4 weeks. The distance of the orthodontic tooth movement into the bovine bone was significantly reduced versus that of the nonbovine bone controls. BB particles accumulated along the root's pressure side during orthodontic treatment. Despite the osteoclasts’ presence adjacent to the BB particles, no BB resorption was observed. Increased RANKL expression was seen at the orthodontic tooth movement pressure zone, without any change in OPG expression. Conclusion: The two novel mouse models show that the lack of resorption of BB xenografts renders them inadequate for proper orthodontic tooth movement at a later stage.

KW - bone regeneration

KW - orthodontics

KW - wound healing

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ER -

Bone regeneration with bovine bone impairs orthodontic tooth movement despite proper osseous wound healing in a novel mouse model

Abstract

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Keywords

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