TY - JOUR
T1 - Borrelia persica
T2 - In vitro cultivation and characterization via conventional PCR and multilocus sequence analysis of two strains isolated from a cat and ticks from Israel
AU - Schwarzer, Sandra
AU - Margos, Gabriele
AU - Overzier, Evelyn
AU - Fingerle, Volker
AU - Baneth, Gad
AU - Straubinger, Reinhard K.
N1 - Publisher Copyright:
© 2015 Elsevier GmbH.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Borrelia persica, one of the pathogenic agents of tick-borne relapsing fever, is transmitted by the soft tick Ornithodoros tholozani. It causes infections in humans as well as in animals. In this study, we developed a medium, termed Pettenkofer/LMU Bp, for reliable in vitro cultivation. Cell densities up to 5.2×107 viable cells/ml were achieved over at least 40 passages. The cultivable B. persica strain isolated from a cat was further analyzed by amplification of the flaB gene using conventional PCR. In addition, seven housekeeping genes (clpA, clpX, pepX, pyrG, recG, rplB and uvrA) of this B. persica strain and a second strain isolated out of pooled ticks from Israel were amplified and the phylogenetic relationships among Borrelia species were analyzed. The results of the conventional PCR and the multilocus sequence analysis confirmed our isolates as B. persica.
AB - Borrelia persica, one of the pathogenic agents of tick-borne relapsing fever, is transmitted by the soft tick Ornithodoros tholozani. It causes infections in humans as well as in animals. In this study, we developed a medium, termed Pettenkofer/LMU Bp, for reliable in vitro cultivation. Cell densities up to 5.2×107 viable cells/ml were achieved over at least 40 passages. The cultivable B. persica strain isolated from a cat was further analyzed by amplification of the flaB gene using conventional PCR. In addition, seven housekeeping genes (clpA, clpX, pepX, pyrG, recG, rplB and uvrA) of this B. persica strain and a second strain isolated out of pooled ticks from Israel were amplified and the phylogenetic relationships among Borrelia species were analyzed. The results of the conventional PCR and the multilocus sequence analysis confirmed our isolates as B. persica.
KW - Borrelia persica
KW - In vitro cultivation
KW - MLSA
KW - MLST
KW - PCR
KW - Relapsing fever spirochete
UR - http://www.scopus.com/inward/record.url?scp=84942365220&partnerID=8YFLogxK
U2 - 10.1016/j.ttbdis.2015.06.012
DO - 10.1016/j.ttbdis.2015.06.012
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C2 - 26169028
AN - SCOPUS:84942365220
SN - 1877-959X
VL - 6
SP - 751
EP - 757
JO - Ticks and Tick-borne Diseases
JF - Ticks and Tick-borne Diseases
IS - 6
ER -
