Bovine Bone Promotes Osseous Protection via Osteoclast Activation

Y. Klein, S. Shani-Kdoshim, A. Maimon, O. Fleissig, O. Levin-Talmor, Y. Meirow, J. Garber-Berkstein, A. Leibovich, A. Stabholz, S. Chaushu, D. Polak*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    4 Scopus citations

    Abstract

    The current study aimed at investigating the long-term biological mechanisms governing bone regeneration in osseous defects filled with bovine bone (BB). Tooth extraction sockets were filled with BB or left unfilled for natural healing in a C57BL/6 mouse alveolar regeneration bone model (n = 12). Seven weeks later, the alveolar bone samples were analyzed histologically with hematoxylin/eosin and tartrate-resistant acid phosphatase staining. A separate group (n = 10) was used for RNA sequencing. Osteoclast inhibition was induced by zoledronic acid (ZA) administration at 2 wk postextraction in a third group (n = 28) for examination of osseous changes and cellular functions with micro–computed tomography and quantitative reverse transcription polymerase chain reaction, respectively. Histological and radiological osseous healing was observed in both BB-filled and normal-healing sockets. However, BB regenerated bone showed significant robust expression of genes associated with bone homeostasis and osteoclasts’ function. Osteoclasts’ inhibition in BB-filled sockets led to decreased bone resorption markers and reduced bone formation to a greater extent than that observed in osteoclasts’ inhibition with natural healing. BB displays long-term biologically active properties, despite a naive osseous histological appearance. These include activation of osteoclasts, which in turn promotes osseous remodeling and maturation of ossified bone.

    Original languageAmerican English
    Pages (from-to)820-829
    Number of pages10
    JournalJournal of Dental Research
    Volume99
    Issue number7
    DOIs
    StatePublished - 1 Jul 2020

    Bibliographical note

    Publisher Copyright:
    © International & American Associations for Dental Research 2020.

    Keywords

    • bone regeneration
    • bone remodeling
    • gene expression
    • histological healing
    • osteoclastogenesis
    • tissue healing

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