Bright/Arid3A acts as a barrier to somatic cell reprogramming through direct regulation of Oct4, Sox2, and Nanog

Melissa Popowski; Troy D. Templeton; Bum Kyu Lee; Catherine Rhee; He Li; Cathrine Miner; Joseph D. Dekker; Shari Orlanski; Yehudit Bergman; Vishwanath R. Iyer; Carol F. Webb; Haley Tucker

doi:10.1016/j.stemcr.2013.12.002

Bright/Arid3A acts as a barrier to somatic cell reprogramming through direct regulation of Oct4, Sox2, and Nanog

Melissa Popowski, Troy D. Templeton, Bum Kyu Lee, Catherine Rhee, He Li, Cathrine Miner, Joseph D. Dekker, Shari Orlanski, Yehudit Bergman, Vishwanath R. Iyer, Carol F. Webb, Haley Tucker^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

We show here that singular loss of the Bright/Arid3A transcription factor leads to reprograming of mouse embryonic fibroblasts (MEFs) and enhancement of standard four-factor (4F) reprogramming. Bright-deficient MEFs bypass senescence and, under standard embryonic stem cell (ESC) culture conditions, spontaneously form clones that in vitro express pluripotency markers, differentiate to all germ lineages, and in vivo form teratomas and chimeric mice. We demonstrate that BRIGHT binds directly to the promoter/enhancer regions of Oct4, Sox2, and Nanog to contribute to their repression in both MEFs and ESCs. Thus, elimination of the BRIGHT barrier may provide an approach for somatic cell reprogramming.

Original language	English
Pages (from-to)	26-35
Number of pages	10
Journal	Stem Cell Reports
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.stemcr.2013.12.002
State	Published - 14 Jan 2014

Bibliographical note

Funding Information:
This work was funded by grants from the NIH (CA-31534, NHARP-003658-0149), Cancer Prevention Research Institute (CPRIT RP100612), and the Maria Betzner Morrow Endowment to H.T. and by an Oklahoma Center for Adult Stem Cell Research award to C.F.W. M.P. is a CPRIT scholar (CPRIT RP101501). We thank Chhaya Das, June Harriss, Maya Ghosh, Amrita Das, Charles Martin, Hongxia Liu, and Stephen Chiang for technical assistance and Jonghwan Kim for discussion and critical reading of the manuscript. We thank the CPRIT Core Facility Support group (CPRIT-RP120348) for assistance with ChIP sequencing library and sequencing.

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title = "Bright/Arid3A acts as a barrier to somatic cell reprogramming through direct regulation of Oct4, Sox2, and Nanog",

abstract = "We show here that singular loss of the Bright/Arid3A transcription factor leads to reprograming of mouse embryonic fibroblasts (MEFs) and enhancement of standard four-factor (4F) reprogramming. Bright-deficient MEFs bypass senescence and, under standard embryonic stem cell (ESC) culture conditions, spontaneously form clones that in vitro express pluripotency markers, differentiate to all germ lineages, and in vivo form teratomas and chimeric mice. We demonstrate that BRIGHT binds directly to the promoter/enhancer regions of Oct4, Sox2, and Nanog to contribute to their repression in both MEFs and ESCs. Thus, elimination of the BRIGHT barrier may provide an approach for somatic cell reprogramming.",

author = "Melissa Popowski and Templeton, {Troy D.} and Lee, {Bum Kyu} and Catherine Rhee and He Li and Cathrine Miner and Dekker, {Joseph D.} and Shari Orlanski and Yehudit Bergman and Iyer, {Vishwanath R.} and Webb, {Carol F.} and Haley Tucker",

note = "Funding Information: This work was funded by grants from the NIH (CA-31534, NHARP-003658-0149), Cancer Prevention Research Institute (CPRIT RP100612), and the Maria Betzner Morrow Endowment to H.T. and by an Oklahoma Center for Adult Stem Cell Research award to C.F.W. M.P. is a CPRIT scholar (CPRIT RP101501). We thank Chhaya Das, June Harriss, Maya Ghosh, Amrita Das, Charles Martin, Hongxia Liu, and Stephen Chiang for technical assistance and Jonghwan Kim for discussion and critical reading of the manuscript. We thank the CPRIT Core Facility Support group (CPRIT-RP120348) for assistance with ChIP sequencing library and sequencing. ",

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AU - Templeton, Troy D.

AU - Lee, Bum Kyu

AU - Rhee, Catherine

AU - Li, He

AU - Miner, Cathrine

AU - Dekker, Joseph D.

AU - Orlanski, Shari

AU - Bergman, Yehudit

AU - Iyer, Vishwanath R.

AU - Webb, Carol F.

AU - Tucker, Haley

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PY - 2014/1/14

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N2 - We show here that singular loss of the Bright/Arid3A transcription factor leads to reprograming of mouse embryonic fibroblasts (MEFs) and enhancement of standard four-factor (4F) reprogramming. Bright-deficient MEFs bypass senescence and, under standard embryonic stem cell (ESC) culture conditions, spontaneously form clones that in vitro express pluripotency markers, differentiate to all germ lineages, and in vivo form teratomas and chimeric mice. We demonstrate that BRIGHT binds directly to the promoter/enhancer regions of Oct4, Sox2, and Nanog to contribute to their repression in both MEFs and ESCs. Thus, elimination of the BRIGHT barrier may provide an approach for somatic cell reprogramming.

AB - We show here that singular loss of the Bright/Arid3A transcription factor leads to reprograming of mouse embryonic fibroblasts (MEFs) and enhancement of standard four-factor (4F) reprogramming. Bright-deficient MEFs bypass senescence and, under standard embryonic stem cell (ESC) culture conditions, spontaneously form clones that in vitro express pluripotency markers, differentiate to all germ lineages, and in vivo form teratomas and chimeric mice. We demonstrate that BRIGHT binds directly to the promoter/enhancer regions of Oct4, Sox2, and Nanog to contribute to their repression in both MEFs and ESCs. Thus, elimination of the BRIGHT barrier may provide an approach for somatic cell reprogramming.

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Bright/Arid3A acts as a barrier to somatic cell reprogramming through direct regulation of Oct4, Sox2, and Nanog

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