Brinker requires two corepressors for maximal and versatile repression in Dpp signalling

Peleg Hasson, Bruno Müller, Konrad Basler, Ze'ev Paroush*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


decapentaplegic (dpp) encodes a Drosophila transforming growth factor-β homologue that functions as a morphogen in the developing embryo and in adult appendage formation. In the wing imaginal disc, a Dpp gradient governs patterning along the antero-posterior axis by inducing regional expression of diverse genes in a concentration-dependent manner. Recent studies show that responses to graded Dpp activity also require an input from a complementary and opposing gradient of Brinker (Brk), a transcriptional repressor protein encoded by a Dpp target gene. Here we show that Brk harbours a functional and transferable repression domain, through which it recruits the corepressors Groucho and CtBP. By analysing transcriptional outcomes arising from the genetic removal of these corepressors, and by ectopically expressing Brk variants in the embryo, we demonstrate that these corepressors are alternatively used by Brk for repressing some Dpp-responsive genes, whereas for repressing other distinct target genes they are not required. Our results show that Brk utilizes multiple means to repress its endogenous target genes, allowing repression of a multitude of complex Dpp target promoters.

Original languageAmerican English
Pages (from-to)5725-5736
Number of pages12
JournalEMBO Journal
Issue number20
StatePublished - 15 Oct 2001


  • Brinker
  • CtBP
  • Dpp signalling
  • Groucho
  • Transcriptional repression


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