C3-Activating proteases on human lymphoblastoid cells superinfected with epstein-barr virus

Thomas Schulz, Manfred P. Dierich*, Eitan Yefenof, George Klein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Mouse spleen lymphocytes to which uncleaved human C3 had been attached were interacted with two EBV- lines (RAMOS and BJAB) and six EBV+ sublines (A.W-RAMOS, 11.W.A.RAMOS, EHR.A.RAMOS, EHR.B.RAMOS; GC-BJAB, BJAB/HRIK), which were obtained by superinfection of the EBV- lines with EBV. Depending on the presence of C3, the EBV- sublines except for EHR-A.RAMOS formed rosettes with the mouse spleen lymphocytes. The interaction was inhibitable by DFP. The experiments are interpreted as indicating the presence of proteolytic, C3-cleaving activity on the surface of the EBV+ sublines. The expression of this activity on the EBV+ lines only, correlates it to the transformation of EBV- lines into EBV+ lines.

Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalCellular Immunology
Volume51
Issue number1
DOIs
StatePublished - Apr 1980
Externally publishedYes

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