TY - JOUR
T1 - Caffeine-induced transmitter release is mediated via ryanodine-sensitive channel
AU - Avidor, Tomer
AU - Clementi, Emilio
AU - Schwartz, Lydia
AU - Atlas, Daphne
PY - 1994/1/3
Y1 - 1994/1/3
N2 - An isolated clone PC12-37 of rat pheochromocytoma PC12 cells, which lacks ryanodine-sensitive Ca2+ channel, responds to depolarization and to agonist activation and triggers [3H]dopamine (3H]DA) release. A caffeine-stimulated transmitter release, while present in the parental PC12 cell line, is completely abolished in PC12-37 cells. In contrast, caffeine-induced Ca2+ influx in PC12-37 cells is similar to that observed in PC12 cells, indicating that caffeine-induced Ca2+ influx is neither mediated by caffeine-induced Ca2+ release nor contributes to the caffeine-induced secretion. These results show (a) a tight coupling between caffeine activation of a ryanodine-sensitive Ca2+ store and transmitter release, (b) no significant involvement of the ryanodine-sensitive Ca2+ channel in depolarization- and agonist-mediated transmitter release, and (c) exclude a major role for caffeine-mediated Ca2+ entry in the caffeine-activated secretion.
AB - An isolated clone PC12-37 of rat pheochromocytoma PC12 cells, which lacks ryanodine-sensitive Ca2+ channel, responds to depolarization and to agonist activation and triggers [3H]dopamine (3H]DA) release. A caffeine-stimulated transmitter release, while present in the parental PC12 cell line, is completely abolished in PC12-37 cells. In contrast, caffeine-induced Ca2+ influx in PC12-37 cells is similar to that observed in PC12 cells, indicating that caffeine-induced Ca2+ influx is neither mediated by caffeine-induced Ca2+ release nor contributes to the caffeine-induced secretion. These results show (a) a tight coupling between caffeine activation of a ryanodine-sensitive Ca2+ store and transmitter release, (b) no significant involvement of the ryanodine-sensitive Ca2+ channel in depolarization- and agonist-mediated transmitter release, and (c) exclude a major role for caffeine-mediated Ca2+ entry in the caffeine-activated secretion.
KW - Caffeine-induced transmitter release
KW - Caffeine-sensitive Ca store
KW - PC12-37 clone
KW - Ryanodine receptor
UR - http://www.scopus.com/inward/record.url?scp=0028047443&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(94)90727-7
DO - 10.1016/0304-3940(94)90727-7
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C2 - 7912413
AN - SCOPUS:0028047443
SN - 0304-3940
VL - 165
SP - 133
EP - 136
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -