Calculated and experimental spin state of seleno cytochrome p450

Yongying Jiang; Santhosh Sivaramakrishnan; Takahiro Hayashi; Shimrit Cohen; Pierre Moënneloccoz; Sason Shaik; Paul R. De Ortiz Montellano

doi:10.1002/anie.200901485

Calculated and experimental spin state of seleno cytochrome p450

Yongying Jiang^*
, Santhosh Sivaramakrishnan
, Takahiro Hayashi
, Shimrit Cohen
, Pierre Moënneloccoz
, Sason Shaik
, Paul R. De Ortiz Montellano

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A change for the better: The proximal cysteine thiolate in a cytochrome P450 enzyme is replaced by a selenocysteine. The resulting selenolate-ligated CYP119 protein has UV/Vis (see spectrum, ), EPR, and resonance Raman spectra that are similar to those of the native protein ( - ). These results are the first to fully describe a hemoprotein with a selenolate proximal ligand.

Original language	English
Pages (from-to)	7193-7195
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	48
Issue number	39
DOIs	https://doi.org/10.1002/anie.200901485
State	Published - 14 Sep 2009

Keywords

Cytochrome p450
Proteins
Raman spectroscopy
Selenium
Spin states

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10.1002/anie.200901485

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AU - Hayashi, Takahiro

AU - Cohen, Shimrit

AU - Moënneloccoz, Pierre

AU - Shaik, Sason

AU - De Ortiz Montellano, Paul R.

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KW - Cytochrome p450

KW - Proteins

KW - Raman spectroscopy

KW - Selenium

KW - Spin states

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Calculated and experimental spin state of seleno cytochrome p450

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Keywords

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