Caldanaerobacter uzonensis sp. nov., an anaerobic, thermophilic, heterotrophic bacterium isolated from a hot spring

Irina V. Kozina, Ilya V. Kublanov, Tatyana V. Kolganova, Nikolai A. Chernyh, Elizaveta A. Bonch-Osmolovskaya

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Abstract

An anaerobic thermophilic bacterium, strain K67T, was isolated from a terrestrial hot spring of. Uzon Caldera, Kamchatka Peninsula. Analysis of the 16S rRNA gene sequence revealed that the novel isolate belongs to the genus Caldanaerobacter, with 95% 16S rRNA gene sequence similarity to Caldanaerobacter subterraneus subsp. subterraneus SEBR 7858T, suggesting that it represents a novel species of the genus Caldanaerobacter. Strain K67T was characterized as an obligate anaerobe, a thermophile (growth at 50-75 °C; optimum 68-70 °C), a neutrophile (growth at pH 25 °C 4.8-8.0; optimum pH25 °C 6.8) and an obligate organotroph (growth by fermentation of various sugars, peptides and polysaccharides). Major fermentation products were acetate, H2 and CO2; ethanol, lactate and L-alanine were formed in smaller amounts. Thiosulfate stimulated growth and was reduced to hydrogen sulfide. Nitrate, sulfate, sulfite and elemental sulfur were not reduced and did not stimulate growth. Thus, according to the strain's phylogenetic position and phenotypic novelties (lower upper limit of temperature range for growth, the ability to grow on arabinose, the inability to reduce elemental sulfur and the formation of alanine as a minor fermentation product), the novel species Caldanaerobacter uzonensis sp. nov. is proposed, with the type strain K67T (=DSM 18923T =VKM B-2408T).

Original languageEnglish
Pages (from-to)1372-1375
Number of pages4
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume60
Issue number6
DOIs
StatePublished - Jun 2010
Externally publishedYes

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