Carbon source affects PKA-dependent polarity of Neurospora crassa in a CRE-1-dependent and independent manner.

Carmit Ziv*, Rena Gorovits, Oded Yarden

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

A defect in mcb, encoding the cAMP-dependent protein kinase A (PKA) regulatory subunit in Neurospora crassa, which confers an apolar growth phenotype, is accompanied by an increase in PKA activity levels. Both PKA and CRE-1 [a key carbon catabolite repression (CCR) regulator] mediate the cellular response to carbon-source availability. Inactivation of the cre-1 gene resulted in reduced growth rate, abnormal hyphal morphology and altered CCR. Both PKA and CRE-1 affected morphology in a carbon-dependent manner, as fructose suppressed the apolar morphology of the mcb strain and enabled faster growth of the Deltacre-1 mutant. An increase in cre-1 transcript abundance was observed in mcb and a reduction in PKA activity levels was measured in Deltacre-1. CRE-1 is involved in determining PKA-dependent polarity, as an mcb;Deltacre-1 strain displayed partial reestablishment of hyphal polarity. Taken together, our results demonstrate regulatory interactions between PKA and CRE-1 that affect cell polarity in a filamentous fungus.

Original languageAmerican English
Pages (from-to)103-116
Number of pages14
JournalExperimental Mycology
Volume45
Issue number2
DOIs
StatePublished - Feb 2008

Bibliographical note

Funding Information:
We thank S. Seiler and Y. Hadar for their helpful comments and discussions and Yi Liu for providing unpublished data. This research project was supported by The Israel Science Foundation.

Fingerprint

Dive into the research topics of 'Carbon source affects PKA-dependent polarity of Neurospora crassa in a CRE-1-dependent and independent manner.'. Together they form a unique fingerprint.

Cite this