Cardiac microvascular endothelial cells express and release nerve growth factor but not fibroblast growth factor-2

Shimon Lecht; Carola Foerster; Hadar Arien-Zakay; Cezary Marcinkiewicz; Philip Lazarovici; Peter I. Lelkes

doi:10.1007/s11626-009-9267-5

Cardiac microvascular endothelial cells express and release nerve growth factor but not fibroblast growth factor-2

Shimon Lecht, Carola Foerster, Hadar Arien-Zakay, Cezary Marcinkiewicz, Philip Lazarovici^*, Peter I. Lelkes

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Endothelial cells (ECs) from different vascular beds not only display common characteristics but are also quite heterogeneous in terms of expression and secretion of neuro-angiogenic factors, which may help explain some of their distinct physiological roles. We investigated by RT-PCR the gene expression, by PC12 bioassay the neurotropic activity, and by ELISAs the levels of NGF and FGF-2 using conditioned medium collected from cultures of ECs derived from myocardial and cerebral capillaries. While NGF was expressed and released by both cell types, FGF-2 was expressed and released solely by the brain but not heart ECs. Oxygen-glucose deprivation (ischemic) insult blocked NGF secretion from heart and brain ECs and inhibited by 70% the secretion of FGF-2 from brain ECs. We propose that the differential expression of NGF and FGF-2 in heart and brain EC cultures reflect heterogeneity on demand of the microcapillary components and the surrounding microenvironment for a proper tissue-specific homeostasis.

Original language	English
Pages (from-to)	469-476
Number of pages	8
Journal	In Vitro Cellular and Developmental Biology - Animal
Volume	46
Issue number	5
DOIs	https://doi.org/10.1007/s11626-009-9267-5
State	Published - May 2010

Bibliographical note

Funding Information:
Acknowledgments This work was supported in part by a grant-in-aid form the Stein Family Foundations (PL and PIL); PL is affiliated and partially supported by the David R. Bloom Center for Pharmacy; and the Dr. Adolf and Klara Brettler Center for Research in Molecular Pharmacology and Therapeutics at The Hebrew University of Jerusalem, Israel; SL is supported by "Eshkol" fellowship from the Israeli Ministry of Science and Technology.

Keywords

Brain
Endothelial heterogeneity
FGF-2
Heart
NGF

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abstract = "Endothelial cells (ECs) from different vascular beds not only display common characteristics but are also quite heterogeneous in terms of expression and secretion of neuro-angiogenic factors, which may help explain some of their distinct physiological roles. We investigated by RT-PCR the gene expression, by PC12 bioassay the neurotropic activity, and by ELISAs the levels of NGF and FGF-2 using conditioned medium collected from cultures of ECs derived from myocardial and cerebral capillaries. While NGF was expressed and released by both cell types, FGF-2 was expressed and released solely by the brain but not heart ECs. Oxygen-glucose deprivation (ischemic) insult blocked NGF secretion from heart and brain ECs and inhibited by 70% the secretion of FGF-2 from brain ECs. We propose that the differential expression of NGF and FGF-2 in heart and brain EC cultures reflect heterogeneity on demand of the microcapillary components and the surrounding microenvironment for a proper tissue-specific homeostasis.",

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AU - Lazarovici, Philip

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Cardiac microvascular endothelial cells express and release nerve growth factor but not fibroblast growth factor-2

Abstract

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Keywords

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