Casein-derived phosphopeptides disrupt tight junction integrity, and precipitously dry up milk secretion in goats

Avi Shamay, Fira Shapiro, Sameer J. Mabjeesh, Nissim Silanikove*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Mammary involution is triggered by local stimuli, but the precise mechanism has not been defined. Milk stasis accumulate local signals, which makes the tight junctions (TJ) leaky. The aim of the study was to check the hypothesis that casein hydrolyzates (CNH) compromise TJ integrity and dry up milk secretion. A single dose of CNH transiently (12 to 24 h) compromised TJ integrity in the treated udder. This was associated by a transient (12 to 96 h) decline in milk secretion. No such changes were recorded in the contralateral gland that served as a control. Four repeated doses of CNH after each milking caused drastic changes in mammary secretion and composition, which were associated with irreversible cessation of milk secretion within 96 h. No such changes were recorded in goats treated with de-phosphorylated casein (control). We conclude that CNH are the milk-borne factors that cause the disruption of TJ integrity and induction of involution, and that the serine-Ps in the CNHs are essential for the excretion of biological activity.

Original languageAmerican English
Pages (from-to)2707-2719
Number of pages13
JournalLife Sciences
Volume70
Issue number23
DOIs
StatePublished - 26 Apr 2002

Keywords

  • Casein
  • Involution
  • Lactation
  • Mammary gland
  • Secretion
  • Serine-P

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