Ca2+, calmodulin, and protein dephosphorylation are required for GA-induced gene expression in petunia corolla

Yael Leitner-Dagan, David Weiss*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Gibberellic acid (GA3) induces the expression of different genes, including chalcone synthase (chs) and gip, in detached petunia corollas. To initiate a study on gibberellin (GA)-signal transduction in this tissue, we examined the effect of agents that inhibit or promote specific steps in signal-transduction pathways. The calcium chelator 1,2-bis(o-aminophenoxy)ethane N,N,N',N'-tetraacetic acid (BAPTA) had no effect on GA-induced gene expression, while the calcium-channel blocker, ruthenium red (RR), inhibited the activation of the genes. The calmodulin antagonist N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7) inhibited the induction of chs and gip by the hormone, and its analog, N-(6-aminohexyl)-1-naphthalenesulfonamide hydrochloride (W-5), had lower effect. The activation of chs and gip by GA3 was completely blocked by the protein phosphatase inhibitor, okadaic acid (OA), and partially inhibited by the protein kinase inhibitor, 1-(5-isoquinoline-sulfonyl)-2-methylpiperazine dihydrochloride (H-7). We suggest that Ca2+ from intracellular sources, calmodulin and protein dephosphorylation and phosphorylation are involved in GA-induced gene expression in petunia corollas.

Original languageAmerican English
Pages (from-to)116-121
Number of pages6
JournalPhysiologia Plantarum
Volume105
Issue number1
DOIs
StatePublished - Jan 1999

Fingerprint

Dive into the research topics of 'Ca2+, calmodulin, and protein dephosphorylation are required for GA-induced gene expression in petunia corolla'. Together they form a unique fingerprint.

Cite this