TY - JOUR
T1 - Caveolin-1 expression in ovarian carcinoma is MDR1 independent
AU - Davidson, Ben
AU - Goldberg, Iris
AU - Givant-Horwitz, Vered
AU - Nesland, Jahn M.
AU - Berner, Aasmund
AU - Bryne, Magne
AU - Risberg, Bjørn
AU - Kopolovic, Juri
AU - Kristensen, Gunnar B.
AU - Tropé, Claes G.
AU - Van de Putte, Gregg
AU - Reich, Reuven
PY - 2002
Y1 - 2002
N2 - We studied the role of caveolin-1 in tumor progression and prognosis in serous ovarian carcinoma and the association between caveolin-1 and MDR1 expression. The study involved immunohistochemical analysis for caveolin-1 and P-glycoprotein (P-gp) expression in 75 effusions and 90 solid lesions from ovarian and primary peritoneal carcinoma; in situ hybridization for MDR1 messenger RNA (mRNA) expression in 62 effusions and all 90 tumors; and reverse transcription-polymerase chain reaction (RT-PCR) for caveolin-1 mRNA expression in 23 effusions. Immunohistochemical analysis localized caveolin-1 to the cell membrane in 43 effusions and 24 tumors. P-gp membrane expression was detected in 14 effusions and 11 tumors; MDR1 mRNA, in 20 effusions and 30 tumors. Caveolin-1 mRNA was expressed in 19 effusions. Caveolin-1 protein expression showed no association with that of P-gp protein or MDR1 mRNA. The expression of all markers was similar in carcinoma cells in pleural and peritoneal effusions. Caveolin-1 is a novel diagnostic marker for effusions; expression is moderately elevated in tumor cells in effusions, possibly owing to altered signal transduction and metabolism in cancer cells at this site. Expression seems MDR1 independent.
AB - We studied the role of caveolin-1 in tumor progression and prognosis in serous ovarian carcinoma and the association between caveolin-1 and MDR1 expression. The study involved immunohistochemical analysis for caveolin-1 and P-glycoprotein (P-gp) expression in 75 effusions and 90 solid lesions from ovarian and primary peritoneal carcinoma; in situ hybridization for MDR1 messenger RNA (mRNA) expression in 62 effusions and all 90 tumors; and reverse transcription-polymerase chain reaction (RT-PCR) for caveolin-1 mRNA expression in 23 effusions. Immunohistochemical analysis localized caveolin-1 to the cell membrane in 43 effusions and 24 tumors. P-gp membrane expression was detected in 14 effusions and 11 tumors; MDR1 mRNA, in 20 effusions and 30 tumors. Caveolin-1 mRNA was expressed in 19 effusions. Caveolin-1 protein expression showed no association with that of P-gp protein or MDR1 mRNA. The expression of all markers was similar in carcinoma cells in pleural and peritoneal effusions. Caveolin-1 is a novel diagnostic marker for effusions; expression is moderately elevated in tumor cells in effusions, possibly owing to altered signal transduction and metabolism in cancer cells at this site. Expression seems MDR1 independent.
KW - Caveolin
KW - Immunohistochemistry
KW - MRNA in situ hybridization
KW - Multidrug resistance
KW - Reverse transcription-polymerase chain reaction
KW - Serous effusions
UR - http://www.scopus.com/inward/record.url?scp=0036328948&partnerID=8YFLogxK
U2 - 10.1309/U40R-1BN4-6KJ3-BDG3
DO - 10.1309/U40R-1BN4-6KJ3-BDG3
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C2 - 11863219
AN - SCOPUS:0036328948
SN - 0002-9173
VL - 117
SP - 225
EP - 234
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 2
ER -
