CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells

Gin Wen Chang; John Q. Davies; Martin Stacey; Simon Yona; Dawn M.E. Bowdish; Jörg Hamann; Tse Ching Chen; Chun Yen Lin; Siamon Gordon; Hsi Hsien Lin

doi:10.1016/j.bbrc.2006.11.148

CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells

Gin Wen Chang, John Q. Davies, Martin Stacey, Simon Yona, Dawn M.E. Bowdish, Jörg Hamann, Tse Ching Chen, Chun Yen Lin, Siamon Gordon, Hsi Hsien Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

EMR2/CD312 is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. Previously it has been shown to interact with chondroitin sulphate glycosaminoglycans in an isoform-specific manner. Although EMR2 expression has been found to be restricted to human myeloid cells, its expression profile has not yet been systemically characterized. In this report, we show that EMR2 receptor expression is up-regulated during differentiation and maturation of macrophages, and is conversely down-regulated during dendritic cell maturation. We also demonstrate that EMR2 receptor alternative splicing and glycosylation is regulated during myeloid differentiation. In monocytes and macrophages, EMR2 can be specifically up-regulated by LPS and IL-10 via an IL-10-mediated pathway. In inflamed tissues, EMR2 is detected in subpopulations of myeloid cells including macrophages and neutrophils. The results presented here further support the idea that EMR2 plays a role in the migration and adhesion of myeloid cells during cell differentiation, maturation, and activation.

Original language	English
Pages (from-to)	133-138
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	353
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.11.148
State	Published - 2 Feb 2007
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by research grants from the British Heart Foundation (H.-H. Lin), Medical Research Council (S. Gordon), U.K., Chang Gung Memorial Hospital (CMRPG340311 to T.-C. Chen, CMRPG32018 to C.-Y. Lin, and CMRPD33008, CMRPD140131 to H.-H. Lin), and the National Science Council, Taiwan (NSC94-2320-B-182-045 to H.-H. Lin).

Keywords

Adhesion-GPCR
EGF-TM7
EMR2
Myeloid cell

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.bbrc.2006.11.148

Cite this

Chang, G. W., Davies, J. Q., Stacey, M., Yona, S., Bowdish, D. M. E., Hamann, J., Chen, T. C., Lin, C. Y., Gordon, S., & Lin, H. H. (2007). CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells. Biochemical and Biophysical Research Communications, 353(1), 133-138. https://doi.org/10.1016/j.bbrc.2006.11.148

@article{30d5c4d3a886468da0c400ea39f1dd7e,

title = "CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells",

abstract = "EMR2/CD312 is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. Previously it has been shown to interact with chondroitin sulphate glycosaminoglycans in an isoform-specific manner. Although EMR2 expression has been found to be restricted to human myeloid cells, its expression profile has not yet been systemically characterized. In this report, we show that EMR2 receptor expression is up-regulated during differentiation and maturation of macrophages, and is conversely down-regulated during dendritic cell maturation. We also demonstrate that EMR2 receptor alternative splicing and glycosylation is regulated during myeloid differentiation. In monocytes and macrophages, EMR2 can be specifically up-regulated by LPS and IL-10 via an IL-10-mediated pathway. In inflamed tissues, EMR2 is detected in subpopulations of myeloid cells including macrophages and neutrophils. The results presented here further support the idea that EMR2 plays a role in the migration and adhesion of myeloid cells during cell differentiation, maturation, and activation.",

keywords = "Adhesion-GPCR, EGF-TM7, EMR2, Myeloid cell",

author = "Chang, {Gin Wen} and Davies, {John Q.} and Martin Stacey and Simon Yona and Bowdish, {Dawn M.E.} and J{\"o}rg Hamann and Chen, {Tse Ching} and Lin, {Chun Yen} and Siamon Gordon and Lin, {Hsi Hsien}",

note = "Funding Information: This study was supported by research grants from the British Heart Foundation (H.-H. Lin), Medical Research Council (S. Gordon), U.K., Chang Gung Memorial Hospital (CMRPG340311 to T.-C. Chen, CMRPG32018 to C.-Y. Lin, and CMRPD33008, CMRPD140131 to H.-H. Lin), and the National Science Council, Taiwan (NSC94-2320-B-182-045 to H.-H. Lin). ",

year = "2007",

month = feb,

day = "2",

doi = "10.1016/j.bbrc.2006.11.148",

language = "אנגלית",

volume = "353",

pages = "133--138",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

Chang, GW, Davies, JQ, Stacey, M, Yona, S, Bowdish, DME, Hamann, J, Chen, TC, Lin, CY, Gordon, S & Lin, HH 2007, 'CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells', Biochemical and Biophysical Research Communications, vol. 353, no. 1, pp. 133-138. https://doi.org/10.1016/j.bbrc.2006.11.148

TY - JOUR

T1 - CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells

AU - Chang, Gin Wen

AU - Davies, John Q.

AU - Stacey, Martin

AU - Yona, Simon

AU - Bowdish, Dawn M.E.

AU - Hamann, Jörg

AU - Chen, Tse Ching

AU - Lin, Chun Yen

AU - Gordon, Siamon

AU - Lin, Hsi Hsien

N1 - Funding Information: This study was supported by research grants from the British Heart Foundation (H.-H. Lin), Medical Research Council (S. Gordon), U.K., Chang Gung Memorial Hospital (CMRPG340311 to T.-C. Chen, CMRPG32018 to C.-Y. Lin, and CMRPD33008, CMRPD140131 to H.-H. Lin), and the National Science Council, Taiwan (NSC94-2320-B-182-045 to H.-H. Lin).

PY - 2007/2/2

Y1 - 2007/2/2

N2 - EMR2/CD312 is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. Previously it has been shown to interact with chondroitin sulphate glycosaminoglycans in an isoform-specific manner. Although EMR2 expression has been found to be restricted to human myeloid cells, its expression profile has not yet been systemically characterized. In this report, we show that EMR2 receptor expression is up-regulated during differentiation and maturation of macrophages, and is conversely down-regulated during dendritic cell maturation. We also demonstrate that EMR2 receptor alternative splicing and glycosylation is regulated during myeloid differentiation. In monocytes and macrophages, EMR2 can be specifically up-regulated by LPS and IL-10 via an IL-10-mediated pathway. In inflamed tissues, EMR2 is detected in subpopulations of myeloid cells including macrophages and neutrophils. The results presented here further support the idea that EMR2 plays a role in the migration and adhesion of myeloid cells during cell differentiation, maturation, and activation.

AB - EMR2/CD312 is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. Previously it has been shown to interact with chondroitin sulphate glycosaminoglycans in an isoform-specific manner. Although EMR2 expression has been found to be restricted to human myeloid cells, its expression profile has not yet been systemically characterized. In this report, we show that EMR2 receptor expression is up-regulated during differentiation and maturation of macrophages, and is conversely down-regulated during dendritic cell maturation. We also demonstrate that EMR2 receptor alternative splicing and glycosylation is regulated during myeloid differentiation. In monocytes and macrophages, EMR2 can be specifically up-regulated by LPS and IL-10 via an IL-10-mediated pathway. In inflamed tissues, EMR2 is detected in subpopulations of myeloid cells including macrophages and neutrophils. The results presented here further support the idea that EMR2 plays a role in the migration and adhesion of myeloid cells during cell differentiation, maturation, and activation.

KW - Adhesion-GPCR

KW - EGF-TM7

KW - EMR2

KW - Myeloid cell

UR - http://www.scopus.com/inward/record.url?scp=33845644687&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.11.148

DO - 10.1016/j.bbrc.2006.11.148

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 17174274

AN - SCOPUS:33845644687

SN - 0006-291X

VL - 353

SP - 133

EP - 138

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this