CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells

Gin Wen Chang, John Q. Davies, Martin Stacey, Simon Yona, Dawn M.E. Bowdish, Jörg Hamann, Tse Ching Chen, Chun Yen Lin, Siamon Gordon, Hsi Hsien Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

EMR2/CD312 is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. Previously it has been shown to interact with chondroitin sulphate glycosaminoglycans in an isoform-specific manner. Although EMR2 expression has been found to be restricted to human myeloid cells, its expression profile has not yet been systemically characterized. In this report, we show that EMR2 receptor expression is up-regulated during differentiation and maturation of macrophages, and is conversely down-regulated during dendritic cell maturation. We also demonstrate that EMR2 receptor alternative splicing and glycosylation is regulated during myeloid differentiation. In monocytes and macrophages, EMR2 can be specifically up-regulated by LPS and IL-10 via an IL-10-mediated pathway. In inflamed tissues, EMR2 is detected in subpopulations of myeloid cells including macrophages and neutrophils. The results presented here further support the idea that EMR2 plays a role in the migration and adhesion of myeloid cells during cell differentiation, maturation, and activation.

Original languageAmerican English
Pages (from-to)133-138
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume353
Issue number1
DOIs
StatePublished - 2 Feb 2007
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by research grants from the British Heart Foundation (H.-H. Lin), Medical Research Council (S. Gordon), U.K., Chang Gung Memorial Hospital (CMRPG340311 to T.-C. Chen, CMRPG32018 to C.-Y. Lin, and CMRPD33008, CMRPD140131 to H.-H. Lin), and the National Science Council, Taiwan (NSC94-2320-B-182-045 to H.-H. Lin).

Keywords

  • Adhesion-GPCR
  • EGF-TM7
  • EMR2
  • Myeloid cell

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