CD48 Expression on Eosinophils in Nasal Polyps of Chronic Rhinosinusitis Patients

Yara Zoabi; Fidan Rahimli Alekberli; Yael Minai-Fleminger; Ron Eliashar; Francesca Levi-Schaffer

doi:10.1159/000515918

CD48 Expression on Eosinophils in Nasal Polyps of Chronic Rhinosinusitis Patients

Yara Zoabi, Fidan Rahimli Alekberli, Yael Minai-Fleminger, Ron Eliashar, Francesca Levi-Schaffer^*

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Introduction: The pathogenesis of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNPs) is not yet completely understood. Based on current knowledge, the infiltration of mast cells and eosinophils in nasal polyps (NPs) plays an important role. This study aimed to investigate the interplay of asthma and allergy etiopathology in CRSwNPs patients by specifically studying tissue mast cells and eosinophils and the pro-inflammatory marker CD48. Methods: Immunohistochemistry was used to assess eosinophils, mast cells, and CD48 expressing eosinophils infiltrating NPs, and flow cytometry was used to assess surface receptors expression on eosinophils from digested NPs. Results: Immunohistochemical analyses showed that mast cell infiltration in NPs is higher in allergic patients in comparison to nonallergic patients; eosinophils infiltration in asthmatic NPs was significantly elevated in comparison to the nonasthmatic NPs, and membrane CD48 (mCD48) expression on eosinophils infiltrating nonallergic asthmatic NPs was highly elevated in comparison to the other subgroups. Similarly, mCD48 and its high-affinity ligand m2B4's expression on eosinophils from enzymatically digested NPs were significantly higher in nonallergic asthmatics in comparison to allergic asthmatics. Conclusions: Eosinophil infiltration in NPs for asthmatic patients, and mast cell infiltration for allergic patients, may be used as reliable biomarkers for endotyping CRSwNPs. In addition, CD48 in asthmatic patients who developed CRSwNPs could be regarded as a potential target for treatment.

Original language	American English
Pages (from-to)	962-970
Number of pages	9
Journal	International Archives of Allergy and Immunology
Volume	182
Issue number	10
DOIs	https://doi.org/10.1159/000515918
State	Published - 1 Oct 2021

Bibliographical note

Funding Information:
This work was supported in part by the Aimwell Charitable Trust (UK), Emalie Gutterman. Memorial Endowed Fund (USA), Israel Science Foundation (Grant No. 442/18), Israel Ministry of Science & Technology, and Rosetrees Trust (UK). F.L.S. is affiliated with the Adolph and Klara Brettler Medical Research Center at the School of Pharmacy of The Hebrew University of Jerusalem.

Publisher Copyright:
© 2021

Keywords

Allergy
Asthma
CD48
Eosinophil
Nasal polyp
Rhinosinusitis

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10.1159/000515918

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@article{63bdf38b87ff48acbeeeb98fc0eda107,

title = "CD48 Expression on Eosinophils in Nasal Polyps of Chronic Rhinosinusitis Patients",

abstract = "Introduction: The pathogenesis of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNPs) is not yet completely understood. Based on current knowledge, the infiltration of mast cells and eosinophils in nasal polyps (NPs) plays an important role. This study aimed to investigate the interplay of asthma and allergy etiopathology in CRSwNPs patients by specifically studying tissue mast cells and eosinophils and the pro-inflammatory marker CD48. Methods: Immunohistochemistry was used to assess eosinophils, mast cells, and CD48 expressing eosinophils infiltrating NPs, and flow cytometry was used to assess surface receptors expression on eosinophils from digested NPs. Results: Immunohistochemical analyses showed that mast cell infiltration in NPs is higher in allergic patients in comparison to nonallergic patients; eosinophils infiltration in asthmatic NPs was significantly elevated in comparison to the nonasthmatic NPs, and membrane CD48 (mCD48) expression on eosinophils infiltrating nonallergic asthmatic NPs was highly elevated in comparison to the other subgroups. Similarly, mCD48 and its high-affinity ligand m2B4's expression on eosinophils from enzymatically digested NPs were significantly higher in nonallergic asthmatics in comparison to allergic asthmatics. Conclusions: Eosinophil infiltration in NPs for asthmatic patients, and mast cell infiltration for allergic patients, may be used as reliable biomarkers for endotyping CRSwNPs. In addition, CD48 in asthmatic patients who developed CRSwNPs could be regarded as a potential target for treatment.",

keywords = "Allergy, Asthma, CD48, Eosinophil, Nasal polyp, Rhinosinusitis",

author = "Yara Zoabi and {Rahimli Alekberli}, Fidan and Yael Minai-Fleminger and Ron Eliashar and Francesca Levi-Schaffer",

note = "Funding Information: This work was supported in part by the Aimwell Charitable Trust (UK), Emalie Gutterman. Memorial Endowed Fund (USA), Israel Science Foundation (Grant No. 442/18), Israel Ministry of Science & Technology, and Rosetrees Trust (UK). F.L.S. is affiliated with the Adolph and Klara Brettler Medical Research Center at the School of Pharmacy of The Hebrew University of Jerusalem. Publisher Copyright: {\textcopyright} 2021 ",

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doi = "10.1159/000515918",

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AU - Zoabi, Yara

AU - Rahimli Alekberli, Fidan

AU - Minai-Fleminger, Yael

AU - Eliashar, Ron

AU - Levi-Schaffer, Francesca

N1 - Funding Information: This work was supported in part by the Aimwell Charitable Trust (UK), Emalie Gutterman. Memorial Endowed Fund (USA), Israel Science Foundation (Grant No. 442/18), Israel Ministry of Science & Technology, and Rosetrees Trust (UK). F.L.S. is affiliated with the Adolph and Klara Brettler Medical Research Center at the School of Pharmacy of The Hebrew University of Jerusalem. Publisher Copyright: © 2021

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Introduction: The pathogenesis of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNPs) is not yet completely understood. Based on current knowledge, the infiltration of mast cells and eosinophils in nasal polyps (NPs) plays an important role. This study aimed to investigate the interplay of asthma and allergy etiopathology in CRSwNPs patients by specifically studying tissue mast cells and eosinophils and the pro-inflammatory marker CD48. Methods: Immunohistochemistry was used to assess eosinophils, mast cells, and CD48 expressing eosinophils infiltrating NPs, and flow cytometry was used to assess surface receptors expression on eosinophils from digested NPs. Results: Immunohistochemical analyses showed that mast cell infiltration in NPs is higher in allergic patients in comparison to nonallergic patients; eosinophils infiltration in asthmatic NPs was significantly elevated in comparison to the nonasthmatic NPs, and membrane CD48 (mCD48) expression on eosinophils infiltrating nonallergic asthmatic NPs was highly elevated in comparison to the other subgroups. Similarly, mCD48 and its high-affinity ligand m2B4's expression on eosinophils from enzymatically digested NPs were significantly higher in nonallergic asthmatics in comparison to allergic asthmatics. Conclusions: Eosinophil infiltration in NPs for asthmatic patients, and mast cell infiltration for allergic patients, may be used as reliable biomarkers for endotyping CRSwNPs. In addition, CD48 in asthmatic patients who developed CRSwNPs could be regarded as a potential target for treatment.

AB - Introduction: The pathogenesis of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNPs) is not yet completely understood. Based on current knowledge, the infiltration of mast cells and eosinophils in nasal polyps (NPs) plays an important role. This study aimed to investigate the interplay of asthma and allergy etiopathology in CRSwNPs patients by specifically studying tissue mast cells and eosinophils and the pro-inflammatory marker CD48. Methods: Immunohistochemistry was used to assess eosinophils, mast cells, and CD48 expressing eosinophils infiltrating NPs, and flow cytometry was used to assess surface receptors expression on eosinophils from digested NPs. Results: Immunohistochemical analyses showed that mast cell infiltration in NPs is higher in allergic patients in comparison to nonallergic patients; eosinophils infiltration in asthmatic NPs was significantly elevated in comparison to the nonasthmatic NPs, and membrane CD48 (mCD48) expression on eosinophils infiltrating nonallergic asthmatic NPs was highly elevated in comparison to the other subgroups. Similarly, mCD48 and its high-affinity ligand m2B4's expression on eosinophils from enzymatically digested NPs were significantly higher in nonallergic asthmatics in comparison to allergic asthmatics. Conclusions: Eosinophil infiltration in NPs for asthmatic patients, and mast cell infiltration for allergic patients, may be used as reliable biomarkers for endotyping CRSwNPs. In addition, CD48 in asthmatic patients who developed CRSwNPs could be regarded as a potential target for treatment.

KW - Allergy

KW - Asthma

KW - CD48

KW - Eosinophil

KW - Nasal polyp

KW - Rhinosinusitis

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AN - SCOPUS:85105122627

SN - 1018-2438

VL - 182

SP - 962

EP - 970

JO - International Archives of Allergy and Immunology

JF - International Archives of Allergy and Immunology

IS - 10

ER -

CD48 Expression on Eosinophils in Nasal Polyps of Chronic Rhinosinusitis Patients

Abstract

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