Recent studies on the oral, anaerobic, gram-negative bacterium Fusobacterium nucleatum revealed its presence and involvement in colorectal, esophageal and breast cancer. We previously demonstrated that F. nucleatum binds and activates the human inhibitory receptors TIGIT and CEACAM1 leading to inhibition of T and NK cell anti-tumor immunity. CEACAM1 was found to be bound and activated by the fusobacterial trimeric autotransporter adhesin CbpF. Here we report the generation of a recombinant E. coli expressing full-length CbpF that efficiently binds and activates CEACAM1.
Bibliographical noteFunding Information:
This work was supported by the Israel Cancer Research Fund Project grant (GB), the Israel Science Foundation Moked grant and the Israel Ministry of Science and Technology Personalized Medicine grant (GB and OM). AS is supported by the Planning and Budgeting Committee of the Israeli Council for Higher Education. JG is supported by the German Research Foundation (DFG) with a postdoctoral research fellowship.
© Copyright © 2021 Shhadeh, Galaski, Alon-Maimon, Fahoum, Wiener, Slade, Mandelboim and Bachrach.
- F. nucleatum
- NK cells
- trimeric autotransporter adhesin