Cell-based biosensor to report DNA damage in micro- and nanosystems

A. Fendyur; S. Varma; C.T. Lo; J. Voldman

doi:10.1021/ac501412c

Cell-based biosensor to report DNA damage in micro- and nanosystems

A. Fendyur, S. Varma, C.T. Lo, J. Voldman

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Understanding how newly engineered micro- and nanoscale materials and systems that interact with cells impact cell physiology is crucial for the development and ultimate adoption of such technologies. Reports regarding the genotoxic impact of forces applied to cells in such systems that can both directly or indirectly damage DNA emphasize the need for developing facile methods to assess how materials and technologies affect cell physiology. To address this need we have developed a TurboRFP-based DNA damage reporter cell line in NIH-3T3 cells that fluoresce to report genotoxic stress caused by a wide variety of agents, from chemical genotoxic agents to UV-C radiation. Our biosensor was successfully implemented in reporting the genotoxic impact of nanomaterials, demonstrating the ability to assess size dependent geno- and cyto-toxicity. The biosensor cells can be assayed in a high throughput, noninvasive manner, with no need for overly sophisticated equipment or additional reagents. We believe that this open-source biosensor is an important resource for the community of micro- and nanomaterials and systems designers and users who wish to evaluate the impact of systems and materials on cell physiology. © 2014 American Chemical Society.

Original language	English
Pages (from-to)	7598-7605
Number of pages	8
Journal	Anal. Chem.
Volume	86
Issue number	15
DOIs	https://doi.org/10.1021/ac501412c
State	Published - 2014

Bibliographical note

Cited By :10

Export Date: 11 September 2022

CODEN: ANCHA

Correspondence Address: Voldman, J.; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States; email: voldman@mit.edu

Funding details: GM090194

Funding details: National Institute of General Medical Sciences, NIGMS, R01GM090194

References: Toh, Y.C., Zhang, C., Zhang, J., Khong, Y.M., Chang, S., Samper, V.D., Van Noort, D., Yu, H., (2007) Lab Chip, 7, pp. 302-309; Bhabra, G., Sood, A., Fisher, B., Cartwright, L., Saunders, M., Evans, W.H., Surprenant, A., Case, C.P., (2009) Nat. Nanotechnol., 4, pp. 876-883; Hung, P.J., Lee, P.J., Sabounchi, P., Lin, R., Lee, L.P., (2005) Biotechnol. Bioeng., 89, pp. 1-8; Huh, D., Matthews, B.D., Mammoto, A., Montoya-Zavala, M., Hsin, H.Y., Ingber, D.E., (2010) Science, 328, pp. 1662-1668; Grosberg, A., Alford, P.W., McCain, M.L., Parker, K.K., (2011) Lab Chip, 11, pp. 4165-4173; Taff, B.M., Voldman, J., (2005) Anal. Chem., 77, pp. 7976-7983; Fendyur, A., Mazurski, N., Shappir, J., Spira, M.E., (2011) Front. Neuroeng., 4, p. 14; Honegger, T., Scott, M.A., Yanik, M.F., Voldman, J., (2013) Lab Chip, 13, pp. 589-598; Cheng, R., Meng, F., Deng, C., Klok, H.A., Zhong, Z., (2013) Biomaterials, 34, pp. 3647-3657; Demirel, G.B., Von Klitzing, R., (2013) ChemPhysChem, 14, pp. 2833-2840; Lopacinska, J.M., Emneus, J., Dufva, M., (2013) PLoS One, 8, p. 53107; Chen, Y., Qu, K., Zhao, C., Wu, L., Ren, J., Wang, J., Qu, X., (2012) Nat. Commun., 3, p. 1074; Papageorgiou, I., Brown, C., Schins, R., Singh, S., Newson, R., Davis, S., Fisher, J., Case, C.P., (2007) Biomaterials, 28, pp. 2946-2958; Asharani, P.V., Low Kah Mun, G., Hande, M.P., Valiyaveettil, S., (2009) ACS Nano, 3, pp. 279-290; Zhang, X., Rosenstein, B.S., Wang, Y., Lebwohl, M., Wei, H., (1997) Free Radical Biol. Med., 23, pp. 980-985; Willers, H., Dahm-Daphi, J., Powell, S.N., (2004) Br. J. Cancer, 90, pp. 1297-1301; Bertram, C., Hass, R., (2008) Biol. Chem., 389, pp. 211-220; Kultz, D., (2005) Annu. Rev. Physiol., 67, pp. 225-257; Au, S.H., Fobel, R., Desai, S.P., Voldman, J., Wheeler, A.R., (2013) Integr. Biol., 5, pp. 1014-1025; Ruiz-Gomez, M.J., Martinez-Morillo, M., (2009) Electromagn. Biol. Med., 28, pp. 201-214; Ge, J., Wood, D.K., Weingeist, D.M., Prasongtanakij, S., Navasumrit, P., Ruchirawat, M., Engelward, B.P., (2013) Cytometry, Part A, 83, pp. 552-560; Purschke, M., Laubach, H.J., Anderson, R.R., Manstein, D., (2010) J. Invest. Dermatol., 130, pp. 86-92; Lai, H., Singh, N.P., (2004) Environ. Health Perspect., 112, pp. 687-694; Guichard, Y., Schmit, J., Darne, C., Gate, L., Goutet, M., Rousset, D., Rastoix, O., Binet, S., (2012) Ann. Occup. Hyg., 56, pp. 631-644; Mu, Q., Hondow, N.S., Krzeminski, L., Brown, A.P., Jeuken, L.J., Routledge, M.N., (2012) Part. Fibre Toxicol., 9, p. 29; Kim, H.R., Park, Y.J., Shin, D.Y., Oh, S.M., Chung, K.H., (2013) Environ. Health Toxicol., 28, p. 2013003; Chen, X., Schluesener, H.J., (2008) Toxicol. Lett., 176, pp. 1-12; Ellinger-Ziegelbauer, H., Fostel, J.M., Aruga, C., Bauer, D., Boitier, E., Deng, S., Dickinson, D., Aubrecht, J., (2009) Toxicol. Sci., 110, pp. 341-352; Azqueta, A., Collins, A.R., (2013) Arch. Toxicol., 87, pp. 949-968; Kuo, L.J., Yang, L.X., (2008) In Vivo, 22, pp. 305-309; Samanta, S., Dey, P., (2012) Diagn. Cytopathol., 40, pp. 84-90; Smith, A.J., Hancock, M.K., Bi, K., Andrews, J., Harrison, P., Vaughan, T.J., (2012) J. Biomol. Screen., 17, pp. 713-726; Hastwell, P.W., Chai, L.L., Roberts, K.J., Webster, T.W., Harvey, J.S., Rees, R.W., Walmsley, R.M., (2006) Mutat. Res., 607, pp. 160-175; Zager, V., Cemazar, M., Hreljac, I., Lah, T.T., Sersa, G., Filipic, M., (2010) Radiol. Oncol., 44, pp. 42-51; Ventura, A., Meissner, A., Dillon, C.P., McManus, M., Sharp, P.A., Van Parijs, L., Jaenisch, R., Jacks, T., (2004) Proc. Natl. Acad. Sci. U.S.A., 101, pp. 10380-10385; Wood, D.K., Weingeist, D.M., Bhatia, S.N., Engelward, B.P., (2010) Proc. Natl. Acad. Sci. U.S.A., 107, pp. 10008-10013; Zhou, B.B., Elledge, S.J., (2000) Nature, 408, pp. 433-439; Levine, A.J., Hu, W., Feng, Z., (2006) Cell Death Differ., 13, pp. 1027-1036; Harris, S.L., Levine, A.J., (2005) Oncogene, 24, pp. 2899-2908; Waldman, T., Kinzler, K.W., Vogelstein, B., (1995) Cancer Res., 55, pp. 5187-5190; Vogelstein, B., Lane, D., Levine, A.J., (2000) Nature, 408, pp. 307-310; Bunz, F., Dutriaux, A., Lengauer, C., Waldman, T., Zhou, S., Brown, J.P., Sedivy, J.M., Vogelstein, B., (1998) Science, 282, pp. 1497-1501; Moldovan, G.L., Pfander, B., Jentsch, S., (2007) Cell, 129, pp. 665-679; Sitko, J.C., Yeh, B., Kim, M., Zhou, H., Takaesu, G., Yoshimura, A., McBride, W.H., Cacalano, N.A., (2008) Cell. Signal., 20, pp. 2221-2230; Hattinger, C.M., Jochemsen, A.G., Tanke, H.J., Dirks, R.W., (2002) J. Histochem. Cytochem., 50, pp. 81-89; Fei, P., Bernhard, E.J., El-Deiry, W.S., (2002) Cancer Res., 62, pp. 7316-7327; Desai, S.P., Voldman, J., (2011) Integr. Biol., 3, pp. 48-56; Gentiletti, F., Mancini, F., D'Angelo, M., Sacchi, A., Pontecorvi, A., Jochemsen, A.G., Moretti, F., (2002) Oncogene, 21, pp. 867-877; Dkhissi, F., Raynal, S., Jullien, P., Lawrence, D.A., (1999) Oncogene, 18, pp. 703-711; Amundson, S.A., Do, K.T., Vinikoor, L., Koch-Paiz, C.A., Bittner, M.L., Trent, J.M., Meltzer, P., Fornace Jr., A.J., (2005) Oncogene, 24, pp. 4572-4579; Hall, J.R., Kow, E., Nevis, K.R., Lu, C.K., Luce, K.S., Zhong, Q., Cook, J.G., (2007) Mol. Biol. Cell, 18, pp. 3340-3350; Kawaguchi, J., Adachi, S., Yasuda, I., Yamauchi, T., Nakashima, M., Ohno, T., Shimizu, M., Moriwaki, H., (2012) Mol. Cancer, 11, p. 45; Dai, T., Vrahas, M.S., Murray, C.K., Hamblin, M.R., (2012) Expert Rev. Anti-Infect. Ther., 10, pp. 185-195; Mannoor, M.S., Jiang, Z., James, T., Kong, Y.L., Malatesta, K.A., Soboyejo, W.O., Verma, N., McAlpine, M.C., (2013) Nano Lett., 13, pp. 2634-2639; Carlson, C., Hussain, S.M., Schrand, A.M., Braydich-Stolle, L.K., Hess, K.L., Jones, R.L., Schlager, J.J., (2008) J. Phys. Chem. B, 112, pp. 13608-13619; Park, J., Lim, D.H., Lim, H.J., Kwon, T., Choi, J.S., Jeong, S., Choi, I.H., Cheon, J., (2011) Chem. Commun. (Cambridge, U. K.), 47, pp. 4382-4384; Wolf-Goldberg, T., Barbul, A., Ben-Dov, N., Korenstein, R., (2013) Biochim. Biophys. Acta, 1833, pp. 1396-1408; Liu, Y., Zhao, Y., Sun, B., Chen, C., (2012) Acc. Chem. Res., 46, pp. 702-713; Knight, A.W., Little, S., Houck, K., Dix, D., Judson, R., Richard, A., McCarroll, N., Walmsley, R.M., (2009) Regul. Toxicol. Pharmacol., 55 (2), pp. 188-199; Bayat, N., Rajapakse, K., Marinsek-Logar, R., Drobne, D., Cristobal, S., (2014) Nanotoxicology, 8, pp. 363-373

Keywords

Cell culture
Cells
DNA
Nanostructured materials
Nanosystems
Physiology
Cell-based biosensor
Genotoxic agents
Genotoxic stress
High throughput
Materials and technologies
Nano-scale materials
Reporter cell line
UV-C radiations
Biosensors
3T3 cell line
animal
DNA damage
flow cytometry
genetic procedures
mouse
nanotechnology
oxidative stress
Animals
Biosensing Techniques
DNA Damage
Flow Cytometry
Mice
Nanotechnology
NIH 3T3 Cells
Oxidative Stress

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10.1021/ac501412c

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84905674449&doi=10.1021%2fac501412c&partnerID=40&md5=af116cae59e7bc033e28e249d2593ad2

Cite this

@article{ae3f1d9a7ebf45d4abfc2d1a7e5ee3fe,

title = "Cell-based biosensor to report DNA damage in micro- and nanosystems",

abstract = "Understanding how newly engineered micro- and nanoscale materials and systems that interact with cells impact cell physiology is crucial for the development and ultimate adoption of such technologies. Reports regarding the genotoxic impact of forces applied to cells in such systems that can both directly or indirectly damage DNA emphasize the need for developing facile methods to assess how materials and technologies affect cell physiology. To address this need we have developed a TurboRFP-based DNA damage reporter cell line in NIH-3T3 cells that fluoresce to report genotoxic stress caused by a wide variety of agents, from chemical genotoxic agents to UV-C radiation. Our biosensor was successfully implemented in reporting the genotoxic impact of nanomaterials, demonstrating the ability to assess size dependent geno- and cyto-toxicity. The biosensor cells can be assayed in a high throughput, noninvasive manner, with no need for overly sophisticated equipment or additional reagents. We believe that this open-source biosensor is an important resource for the community of micro- and nanomaterials and systems designers and users who wish to evaluate the impact of systems and materials on cell physiology. {\textcopyright} 2014 American Chemical Society.",

keywords = "Cell culture, Cells, DNA, Nanostructured materials, Nanosystems, Physiology, Cell-based biosensor, Genotoxic agents, Genotoxic stress, High throughput, Materials and technologies, Nano-scale materials, Reporter cell line, UV-C radiations, Biosensors, 3T3 cell line, animal, DNA damage, flow cytometry, genetic procedures, mouse, nanotechnology, oxidative stress, Animals, Biosensing Techniques, DNA Damage, Flow Cytometry, Mice, Nanotechnology, NIH 3T3 Cells, Oxidative Stress",

author = "A. Fendyur and S. Varma and C.T. Lo and J. Voldman",

note = "Cited By :10 Export Date: 11 September 2022 CODEN: ANCHA Correspondence Address: Voldman, J.; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States; email: voldman@mit.edu Funding details: GM090194 Funding details: National Institute of General Medical Sciences, NIGMS, R01GM090194 References: Toh, Y.C., Zhang, C., Zhang, J., Khong, Y.M., Chang, S., Samper, V.D., Van Noort, D., Yu, H., (2007) Lab Chip, 7, pp. 302-309; Bhabra, G., Sood, A., Fisher, B., Cartwright, L., Saunders, M., Evans, W.H., Surprenant, A., Case, C.P., (2009) Nat. Nanotechnol., 4, pp. 876-883; Hung, P.J., Lee, P.J., Sabounchi, P., Lin, R., Lee, L.P., (2005) Biotechnol. Bioeng., 89, pp. 1-8; Huh, D., Matthews, B.D., Mammoto, A., Montoya-Zavala, M., Hsin, H.Y., Ingber, D.E., (2010) Science, 328, pp. 1662-1668; Grosberg, A., Alford, P.W., McCain, M.L., Parker, K.K., (2011) Lab Chip, 11, pp. 4165-4173; Taff, B.M., Voldman, J., (2005) Anal. Chem., 77, pp. 7976-7983; Fendyur, A., Mazurski, N., Shappir, J., Spira, M.E., (2011) Front. Neuroeng., 4, p. 14; Honegger, T., Scott, M.A., Yanik, M.F., Voldman, J., (2013) Lab Chip, 13, pp. 589-598; Cheng, R., Meng, F., Deng, C., Klok, H.A., Zhong, Z., (2013) Biomaterials, 34, pp. 3647-3657; Demirel, G.B., Von Klitzing, R., (2013) ChemPhysChem, 14, pp. 2833-2840; Lopacinska, J.M., Emneus, J., Dufva, M., (2013) PLoS One, 8, p. 53107; Chen, Y., Qu, K., Zhao, C., Wu, L., Ren, J., Wang, J., Qu, X., (2012) Nat. Commun., 3, p. 1074; Papageorgiou, I., Brown, C., Schins, R., Singh, S., Newson, R., Davis, S., Fisher, J., Case, C.P., (2007) Biomaterials, 28, pp. 2946-2958; Asharani, P.V., Low Kah Mun, G., Hande, M.P., Valiyaveettil, S., (2009) ACS Nano, 3, pp. 279-290; Zhang, X., Rosenstein, B.S., Wang, Y., Lebwohl, M., Wei, H., (1997) Free Radical Biol. Med., 23, pp. 980-985; Willers, H., Dahm-Daphi, J., Powell, S.N., (2004) Br. J. Cancer, 90, pp. 1297-1301; Bertram, C., Hass, R., (2008) Biol. Chem., 389, pp. 211-220; Kultz, D., (2005) Annu. Rev. Physiol., 67, pp. 225-257; Au, S.H., Fobel, R., Desai, S.P., Voldman, J., Wheeler, A.R., (2013) Integr. Biol., 5, pp. 1014-1025; Ruiz-Gomez, M.J., Martinez-Morillo, M., (2009) Electromagn. Biol. Med., 28, pp. 201-214; Ge, J., Wood, D.K., Weingeist, D.M., Prasongtanakij, S., Navasumrit, P., Ruchirawat, M., Engelward, B.P., (2013) Cytometry, Part A, 83, pp. 552-560; Purschke, M., Laubach, H.J., Anderson, R.R., Manstein, D., (2010) J. Invest. Dermatol., 130, pp. 86-92; Lai, H., Singh, N.P., (2004) Environ. Health Perspect., 112, pp. 687-694; Guichard, Y., Schmit, J., Darne, C., Gate, L., Goutet, M., Rousset, D., Rastoix, O., Binet, S., (2012) Ann. Occup. Hyg., 56, pp. 631-644; Mu, Q., Hondow, N.S., Krzeminski, L., Brown, A.P., Jeuken, L.J., Routledge, M.N., (2012) Part. Fibre Toxicol., 9, p. 29; Kim, H.R., Park, Y.J., Shin, D.Y., Oh, S.M., Chung, K.H., (2013) Environ. Health Toxicol., 28, p. 2013003; Chen, X., Schluesener, H.J., (2008) Toxicol. Lett., 176, pp. 1-12; Ellinger-Ziegelbauer, H., Fostel, J.M., Aruga, C., Bauer, D., Boitier, E., Deng, S., Dickinson, D., Aubrecht, J., (2009) Toxicol. Sci., 110, pp. 341-352; Azqueta, A., Collins, A.R., (2013) Arch. Toxicol., 87, pp. 949-968; Kuo, L.J., Yang, L.X., (2008) In Vivo, 22, pp. 305-309; Samanta, S., Dey, P., (2012) Diagn. Cytopathol., 40, pp. 84-90; Smith, A.J., Hancock, M.K., Bi, K., Andrews, J., Harrison, P., Vaughan, T.J., (2012) J. Biomol. Screen., 17, pp. 713-726; Hastwell, P.W., Chai, L.L., Roberts, K.J., Webster, T.W., Harvey, J.S., Rees, R.W., Walmsley, R.M., (2006) Mutat. Res., 607, pp. 160-175; Zager, V., Cemazar, M., Hreljac, I., Lah, T.T., Sersa, G., Filipic, M., (2010) Radiol. Oncol., 44, pp. 42-51; Ventura, A., Meissner, A., Dillon, C.P., McManus, M., Sharp, P.A., Van Parijs, L., Jaenisch, R., Jacks, T., (2004) Proc. Natl. Acad. Sci. U.S.A., 101, pp. 10380-10385; Wood, D.K., Weingeist, D.M., Bhatia, S.N., Engelward, B.P., (2010) Proc. Natl. Acad. Sci. U.S.A., 107, pp. 10008-10013; Zhou, B.B., Elledge, S.J., (2000) Nature, 408, pp. 433-439; Levine, A.J., Hu, W., Feng, Z., (2006) Cell Death Differ., 13, pp. 1027-1036; Harris, S.L., Levine, A.J., (2005) Oncogene, 24, pp. 2899-2908; Waldman, T., Kinzler, K.W., Vogelstein, B., (1995) Cancer Res., 55, pp. 5187-5190; Vogelstein, B., Lane, D., Levine, A.J., (2000) Nature, 408, pp. 307-310; Bunz, F., Dutriaux, A., Lengauer, C., Waldman, T., Zhou, S., Brown, J.P., Sedivy, J.M., Vogelstein, B., (1998) Science, 282, pp. 1497-1501; Moldovan, G.L., Pfander, B., Jentsch, S., (2007) Cell, 129, pp. 665-679; Sitko, J.C., Yeh, B., Kim, M., Zhou, H., Takaesu, G., Yoshimura, A., McBride, W.H., Cacalano, N.A., (2008) Cell. Signal., 20, pp. 2221-2230; Hattinger, C.M., Jochemsen, A.G., Tanke, H.J., Dirks, R.W., (2002) J. Histochem. Cytochem., 50, pp. 81-89; Fei, P., Bernhard, E.J., El-Deiry, W.S., (2002) Cancer Res., 62, pp. 7316-7327; Desai, S.P., Voldman, J., (2011) Integr. Biol., 3, pp. 48-56; Gentiletti, F., Mancini, F., D'Angelo, M., Sacchi, A., Pontecorvi, A., Jochemsen, A.G., Moretti, F., (2002) Oncogene, 21, pp. 867-877; Dkhissi, F., Raynal, S., Jullien, P., Lawrence, D.A., (1999) Oncogene, 18, pp. 703-711; Amundson, S.A., Do, K.T., Vinikoor, L., Koch-Paiz, C.A., Bittner, M.L., Trent, J.M., Meltzer, P., Fornace Jr., A.J., (2005) Oncogene, 24, pp. 4572-4579; Hall, J.R., Kow, E., Nevis, K.R., Lu, C.K., Luce, K.S., Zhong, Q., Cook, J.G., (2007) Mol. Biol. Cell, 18, pp. 3340-3350; Kawaguchi, J., Adachi, S., Yasuda, I., Yamauchi, T., Nakashima, M., Ohno, T., Shimizu, M., Moriwaki, H., (2012) Mol. Cancer, 11, p. 45; Dai, T., Vrahas, M.S., Murray, C.K., Hamblin, M.R., (2012) Expert Rev. Anti-Infect. Ther., 10, pp. 185-195; Mannoor, M.S., Jiang, Z., James, T., Kong, Y.L., Malatesta, K.A., Soboyejo, W.O., Verma, N., McAlpine, M.C., (2013) Nano Lett., 13, pp. 2634-2639; Carlson, C., Hussain, S.M., Schrand, A.M., Braydich-Stolle, L.K., Hess, K.L., Jones, R.L., Schlager, J.J., (2008) J. Phys. Chem. B, 112, pp. 13608-13619; Park, J., Lim, D.H., Lim, H.J., Kwon, T., Choi, J.S., Jeong, S., Choi, I.H., Cheon, J., (2011) Chem. Commun. (Cambridge, U. K.), 47, pp. 4382-4384; Wolf-Goldberg, T., Barbul, A., Ben-Dov, N., Korenstein, R., (2013) Biochim. Biophys. Acta, 1833, pp. 1396-1408; Liu, Y., Zhao, Y., Sun, B., Chen, C., (2012) Acc. Chem. Res., 46, pp. 702-713; Knight, A.W., Little, S., Houck, K., Dix, D., Judson, R., Richard, A., McCarroll, N., Walmsley, R.M., (2009) Regul. Toxicol. Pharmacol., 55 (2), pp. 188-199; Bayat, N., Rajapakse, K., Marinsek-Logar, R., Drobne, D., Cristobal, S., (2014) Nanotoxicology, 8, pp. 363-373",

year = "2014",

doi = "10.1021/ac501412c",

language = "אנגלית",

volume = "86",

pages = "7598--7605",

journal = "Anal. Chem.",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "15",

}

TY - JOUR

T1 - Cell-based biosensor to report DNA damage in micro- and nanosystems

AU - Fendyur, A.

AU - Varma, S.

AU - Lo, C.T.

AU - Voldman, J.

N1 - Cited By :10 Export Date: 11 September 2022 CODEN: ANCHA Correspondence Address: Voldman, J.; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States; email: voldman@mit.edu Funding details: GM090194 Funding details: National Institute of General Medical Sciences, NIGMS, R01GM090194 References: Toh, Y.C., Zhang, C., Zhang, J., Khong, Y.M., Chang, S., Samper, V.D., Van Noort, D., Yu, H., (2007) Lab Chip, 7, pp. 302-309; Bhabra, G., Sood, A., Fisher, B., Cartwright, L., Saunders, M., Evans, W.H., Surprenant, A., Case, C.P., (2009) Nat. Nanotechnol., 4, pp. 876-883; Hung, P.J., Lee, P.J., Sabounchi, P., Lin, R., Lee, L.P., (2005) Biotechnol. Bioeng., 89, pp. 1-8; Huh, D., Matthews, B.D., Mammoto, A., Montoya-Zavala, M., Hsin, H.Y., Ingber, D.E., (2010) Science, 328, pp. 1662-1668; Grosberg, A., Alford, P.W., McCain, M.L., Parker, K.K., (2011) Lab Chip, 11, pp. 4165-4173; Taff, B.M., Voldman, J., (2005) Anal. Chem., 77, pp. 7976-7983; Fendyur, A., Mazurski, N., Shappir, J., Spira, M.E., (2011) Front. Neuroeng., 4, p. 14; Honegger, T., Scott, M.A., Yanik, M.F., Voldman, J., (2013) Lab Chip, 13, pp. 589-598; Cheng, R., Meng, F., Deng, C., Klok, H.A., Zhong, Z., (2013) Biomaterials, 34, pp. 3647-3657; Demirel, G.B., Von Klitzing, R., (2013) ChemPhysChem, 14, pp. 2833-2840; Lopacinska, J.M., Emneus, J., Dufva, M., (2013) PLoS One, 8, p. 53107; Chen, Y., Qu, K., Zhao, C., Wu, L., Ren, J., Wang, J., Qu, X., (2012) Nat. 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PY - 2014

Y1 - 2014

N2 - Understanding how newly engineered micro- and nanoscale materials and systems that interact with cells impact cell physiology is crucial for the development and ultimate adoption of such technologies. Reports regarding the genotoxic impact of forces applied to cells in such systems that can both directly or indirectly damage DNA emphasize the need for developing facile methods to assess how materials and technologies affect cell physiology. To address this need we have developed a TurboRFP-based DNA damage reporter cell line in NIH-3T3 cells that fluoresce to report genotoxic stress caused by a wide variety of agents, from chemical genotoxic agents to UV-C radiation. Our biosensor was successfully implemented in reporting the genotoxic impact of nanomaterials, demonstrating the ability to assess size dependent geno- and cyto-toxicity. The biosensor cells can be assayed in a high throughput, noninvasive manner, with no need for overly sophisticated equipment or additional reagents. We believe that this open-source biosensor is an important resource for the community of micro- and nanomaterials and systems designers and users who wish to evaluate the impact of systems and materials on cell physiology. © 2014 American Chemical Society.

AB - Understanding how newly engineered micro- and nanoscale materials and systems that interact with cells impact cell physiology is crucial for the development and ultimate adoption of such technologies. Reports regarding the genotoxic impact of forces applied to cells in such systems that can both directly or indirectly damage DNA emphasize the need for developing facile methods to assess how materials and technologies affect cell physiology. To address this need we have developed a TurboRFP-based DNA damage reporter cell line in NIH-3T3 cells that fluoresce to report genotoxic stress caused by a wide variety of agents, from chemical genotoxic agents to UV-C radiation. Our biosensor was successfully implemented in reporting the genotoxic impact of nanomaterials, demonstrating the ability to assess size dependent geno- and cyto-toxicity. The biosensor cells can be assayed in a high throughput, noninvasive manner, with no need for overly sophisticated equipment or additional reagents. We believe that this open-source biosensor is an important resource for the community of micro- and nanomaterials and systems designers and users who wish to evaluate the impact of systems and materials on cell physiology. © 2014 American Chemical Society.

KW - Cell culture

KW - Cells

KW - DNA

KW - Nanostructured materials

KW - Nanosystems

KW - Physiology

KW - Cell-based biosensor

KW - Genotoxic agents

KW - Genotoxic stress

KW - High throughput

KW - Materials and technologies

KW - Nano-scale materials

KW - Reporter cell line

KW - UV-C radiations

KW - Biosensors

KW - 3T3 cell line

KW - animal

KW - DNA damage

KW - flow cytometry

KW - genetic procedures

KW - mouse

KW - nanotechnology

KW - oxidative stress

KW - Animals

KW - Biosensing Techniques

KW - DNA Damage

KW - Flow Cytometry

KW - Mice

KW - Nanotechnology

KW - NIH 3T3 Cells

KW - Oxidative Stress

UR - http://www.scopus.com/inward/record.url?scp=84905674449&partnerID=8YFLogxK

U2 - 10.1021/ac501412c

DO - 10.1021/ac501412c

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

SN - 0003-2700

VL - 86

SP - 7598

EP - 7605

JO - Anal. Chem.

JF - Anal. Chem.

IS - 15

ER -

Cell-based biosensor to report DNA damage in micro- and nanosystems

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