TY - JOUR
T1 - Cell-cycle research with synchronous cultures
T2 - An evaluation
AU - Helmstetter, Charles E.
AU - Thornton, Maureen
AU - Grover, N. B.
PY - 2001
Y1 - 2001
N2 - The baby-machine system, which produces new-born Escherichia coli cells from cultures immobilized on a membrane, was developed many years ago in an attempt to attain optimal synchrony with minimal disturbance of steady-state growth. In the present article, we put forward a model to describe the behaviour of cells produced by this method, and provide quantitative evaluation of the parameters involved, at each of four different growth rates. Considering the high level of selection achievable with this technique and the natural dispersion in interdivision times, we believe that the output of the baby machine is probably close to optimal in terms of both quality and persistence of synchrony. We show that considerable information on events in the cell cycle can be obtained from populations with age distributions very much broader than those achieved with the baby machine and differing only modestly from steady state. The data presented here, together with the long and fruitful history of findings employing the baby-machine technique, suggest that minimisation of stress on cells is the single most important factor for successful cell-cycle analysis.
AB - The baby-machine system, which produces new-born Escherichia coli cells from cultures immobilized on a membrane, was developed many years ago in an attempt to attain optimal synchrony with minimal disturbance of steady-state growth. In the present article, we put forward a model to describe the behaviour of cells produced by this method, and provide quantitative evaluation of the parameters involved, at each of four different growth rates. Considering the high level of selection achievable with this technique and the natural dispersion in interdivision times, we believe that the output of the baby machine is probably close to optimal in terms of both quality and persistence of synchrony. We show that considerable information on events in the cell cycle can be obtained from populations with age distributions very much broader than those achieved with the baby machine and differing only modestly from steady state. The data presented here, together with the long and fruitful history of findings employing the baby-machine technique, suggest that minimisation of stress on cells is the single most important factor for successful cell-cycle analysis.
KW - Baby machine
KW - Cell cycle
KW - Distribution of cell interdivision times
KW - Model for synchronous growth of Escherichia coli
KW - Synchronous growth parameters
UR - http://www.scopus.com/inward/record.url?scp=0035114288&partnerID=8YFLogxK
U2 - 10.1016/S0300-9084(00)01229-3
DO - 10.1016/S0300-9084(00)01229-3
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.systematicreview???
C2 - 11254979
AN - SCOPUS:0035114288
SN - 0300-9084
VL - 83
SP - 83
EP - 89
JO - Biochimie
JF - Biochimie
IS - 1
ER -