TY - JOUR
T1 - Cell division is not a "clock" measuring acquisition of competence to produce IFN-γ or IL-4
AU - Ben-Sasson, S. Z.
AU - Gerstel, R.
AU - Hu-Li, J.
AU - Paul, W. E.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Naive CD4 T cells acquire the potential to produce IFN-γ and IL-4 by culture in the presence of their cognate Ag, APC, and appropriate cytokines. In this study, we show that commitment to IFN-γ production on the part of rigorously purified naive CD4 T tells can occur without cell division. Indeed, even entry into S phase is not essential. Moreover, both CD4 and CD4/CD8 thymocytes from TCR-transgenic mice (5CC7 mice) on a Rag2-/- background can acquire IFN-γ-producing capacity when stimulated by peptide, APC, and IL-12. These cells can do so without dividing and some acquire IFN-γ-producing activity without entry into S phase. Not only is cell division not required for acquisition of cytokine-producing potential, cell populations that have undergone the same numbers of divisions can have quite different proportions of IFN-γ- or IL-4-producing cells, depending on the duration of priming or, in the case of IL-4, on the concentration of peptide. Thus, cell division is not a clock for the expression of these cytokines. Factors associated with priming conditions including strength of stimulation, duration of priming, and number of divisions each play a role.
AB - Naive CD4 T cells acquire the potential to produce IFN-γ and IL-4 by culture in the presence of their cognate Ag, APC, and appropriate cytokines. In this study, we show that commitment to IFN-γ production on the part of rigorously purified naive CD4 T tells can occur without cell division. Indeed, even entry into S phase is not essential. Moreover, both CD4 and CD4/CD8 thymocytes from TCR-transgenic mice (5CC7 mice) on a Rag2-/- background can acquire IFN-γ-producing capacity when stimulated by peptide, APC, and IL-12. These cells can do so without dividing and some acquire IFN-γ-producing activity without entry into S phase. Not only is cell division not required for acquisition of cytokine-producing potential, cell populations that have undergone the same numbers of divisions can have quite different proportions of IFN-γ- or IL-4-producing cells, depending on the duration of priming or, in the case of IL-4, on the concentration of peptide. Thus, cell division is not a clock for the expression of these cytokines. Factors associated with priming conditions including strength of stimulation, duration of priming, and number of divisions each play a role.
UR - http://www.scopus.com/inward/record.url?scp=0035167553&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.166.1.112
DO - 10.4049/jimmunol.166.1.112
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C2 - 11123283
AN - SCOPUS:0035167553
SN - 0022-1767
VL - 166
SP - 112
EP - 120
JO - Journal of Immunology
JF - Journal of Immunology
IS - 1
ER -