Cell division is not a "clock" measuring acquisition of competence to produce IFN-γ or IL-4

S. Z. Ben-Sasson, R. Gerstel, J. Hu-Li, W. E. Paul*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Naive CD4 T cells acquire the potential to produce IFN-γ and IL-4 by culture in the presence of their cognate Ag, APC, and appropriate cytokines. In this study, we show that commitment to IFN-γ production on the part of rigorously purified naive CD4 T tells can occur without cell division. Indeed, even entry into S phase is not essential. Moreover, both CD4 and CD4/CD8 thymocytes from TCR-transgenic mice (5CC7 mice) on a Rag2-/- background can acquire IFN-γ-producing capacity when stimulated by peptide, APC, and IL-12. These cells can do so without dividing and some acquire IFN-γ-producing activity without entry into S phase. Not only is cell division not required for acquisition of cytokine-producing potential, cell populations that have undergone the same numbers of divisions can have quite different proportions of IFN-γ- or IL-4-producing cells, depending on the duration of priming or, in the case of IL-4, on the concentration of peptide. Thus, cell division is not a clock for the expression of these cytokines. Factors associated with priming conditions including strength of stimulation, duration of priming, and number of divisions each play a role.

Original languageEnglish
Pages (from-to)112-120
Number of pages9
JournalJournal of Immunology
Volume166
Issue number1
DOIs
StatePublished - 1 Jan 2001
Externally publishedYes

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