Cell wall synthesis and initiation of deoxyribonucleic acid replication in Bacillus subtilis

N. Sandler, A. Keynan

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The authors have observed a connection between cell wall synthesis and the initiation of chromosome replication in B. subtilis. Initiation of chromosome replication was prevented in synchronous cultures in the presence of the cell wall synthesis inhibitor vancomycin. When vancomycin was added to the cultures after initiation of chromosome replication, one round of replication was completed but no reinitiation occurred. Similar results were obtained when cell wall synthesis was inhibited by ristocetin, cycloserine, cloxacillin, or cephaloridine. When sucrose was added to the medium, initiation of deoxyribonucleic acid replication occurred in the presence of vancomycin, to an extent allowed replication of no more than approximately one-half of the deoxyribonucleic acid of the culture. The same was found in cultures of spheroplasts of B. subtilis. However, initiation of chromosome replication in spheroplasts was completely insensitive to cloxacillin.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalJournal of Bacteriology
Volume148
Issue number2
StatePublished - 1981

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