TY - JOUR
T1 - Cell wall synthesis and initiation of deoxyribonucleic acid replication in Bacillus subtilis
AU - Sandler, N.
AU - Keynan, A.
PY - 1981
Y1 - 1981
N2 - The authors have observed a connection between cell wall synthesis and the initiation of chromosome replication in B. subtilis. Initiation of chromosome replication was prevented in synchronous cultures in the presence of the cell wall synthesis inhibitor vancomycin. When vancomycin was added to the cultures after initiation of chromosome replication, one round of replication was completed but no reinitiation occurred. Similar results were obtained when cell wall synthesis was inhibited by ristocetin, cycloserine, cloxacillin, or cephaloridine. When sucrose was added to the medium, initiation of deoxyribonucleic acid replication occurred in the presence of vancomycin, to an extent allowed replication of no more than approximately one-half of the deoxyribonucleic acid of the culture. The same was found in cultures of spheroplasts of B. subtilis. However, initiation of chromosome replication in spheroplasts was completely insensitive to cloxacillin.
AB - The authors have observed a connection between cell wall synthesis and the initiation of chromosome replication in B. subtilis. Initiation of chromosome replication was prevented in synchronous cultures in the presence of the cell wall synthesis inhibitor vancomycin. When vancomycin was added to the cultures after initiation of chromosome replication, one round of replication was completed but no reinitiation occurred. Similar results were obtained when cell wall synthesis was inhibited by ristocetin, cycloserine, cloxacillin, or cephaloridine. When sucrose was added to the medium, initiation of deoxyribonucleic acid replication occurred in the presence of vancomycin, to an extent allowed replication of no more than approximately one-half of the deoxyribonucleic acid of the culture. The same was found in cultures of spheroplasts of B. subtilis. However, initiation of chromosome replication in spheroplasts was completely insensitive to cloxacillin.
UR - http://www.scopus.com/inward/record.url?scp=0019773933&partnerID=8YFLogxK
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C2 - 6795178
AN - SCOPUS:0019773933
SN - 0021-9193
VL - 148
SP - 443
EP - 449
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 2
ER -