Challenging semi-bootstrapping molecular-replacement strategy reveals intriguing crystal packing of rhizavidin

Amit Meir, Oded Livnah*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The structure of rhizavidin, the first dimeric member of the avidin family which maintains high affinity towards biotin, was determined to high resolution by SeMet SAD. Consequently, the structure of the rhizavidin-biotin complex was determined by molecular-replacement methods using the apo structure as the search model; this ran into complications and required combined programs as well as bootstrapping approaches. Although present as a dimer in solution, rhizavidin packs as unique oligomers in both crystal forms. The novel insights derived from the unique molecular-replacement procedure and the crystal-driven oligomeric forms in this work may have utililty in biotechological and nanotechnological applications.

Original languageAmerican English
Pages (from-to)373-378
Number of pages6
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number4
DOIs
StatePublished - 2010

Keywords

  • Crystal packing
  • Molecular replacement
  • Rhizavidin

Fingerprint

Dive into the research topics of 'Challenging semi-bootstrapping molecular-replacement strategy reveals intriguing crystal packing of rhizavidin'. Together they form a unique fingerprint.

Cite this