TY - JOUR
T1 - Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model
AU - Barliya, Tilda
AU - Ofri, Ron
AU - Sandalon, Shai
AU - Weinberger, Dov
AU - Livnat, Tami
N1 - Publisher Copyright:
© 2017 Tilda Barliya et al.
PY - 2017
Y1 - 2017
N2 - Purpose. To explore functional electroretinographic (ERG) changes and associated cellular remodeling following experimental retinal detachment in a rabbit model. Methods. Retinal detachment was created in ten rabbits by injecting 0.1 ml balanced salt solution under the retina. Fundus imaging was performed 0, 3, 7, 14, and 21 days postoperatively. ERGs were recorded pre- A nd 7 and 21 days postoperatively. Eyes were harvested on day 21 and evaluated immunohistochemically (IHC) for remodeling of second- A nd third-order neurons. Results. Retinal reattachment occurred within two weeks following surgery. No attenuation was observed in the photopic or scotopic a- A nd b-waves. A secondary wavefront on the descending slope of the scotopic b-wave was the only ERG result that was attenuated in detached retinas. IHC demonstrated anatomical changes in both ON and OFF bipolar cells. Bassoon staining was observed in the remodeled dendrites. Amacrine and horizontal cells did not alter, but Muller cells were clearly reactive with marked extension. Conclusion. Retinal detachment and reattachment were associated with functional and anatomical changes. Exploring the significance of the secondary scotopic wavefront and its association with the remodeling of 2nd- A nd 3rd-order neurons will shade more light on functional changes and recovery of the retina.
AB - Purpose. To explore functional electroretinographic (ERG) changes and associated cellular remodeling following experimental retinal detachment in a rabbit model. Methods. Retinal detachment was created in ten rabbits by injecting 0.1 ml balanced salt solution under the retina. Fundus imaging was performed 0, 3, 7, 14, and 21 days postoperatively. ERGs were recorded pre- A nd 7 and 21 days postoperatively. Eyes were harvested on day 21 and evaluated immunohistochemically (IHC) for remodeling of second- A nd third-order neurons. Results. Retinal reattachment occurred within two weeks following surgery. No attenuation was observed in the photopic or scotopic a- A nd b-waves. A secondary wavefront on the descending slope of the scotopic b-wave was the only ERG result that was attenuated in detached retinas. IHC demonstrated anatomical changes in both ON and OFF bipolar cells. Bassoon staining was observed in the remodeled dendrites. Amacrine and horizontal cells did not alter, but Muller cells were clearly reactive with marked extension. Conclusion. Retinal detachment and reattachment were associated with functional and anatomical changes. Exploring the significance of the secondary scotopic wavefront and its association with the remodeling of 2nd- A nd 3rd-order neurons will shade more light on functional changes and recovery of the retina.
UR - http://www.scopus.com/inward/record.url?scp=85018854641&partnerID=8YFLogxK
U2 - 10.1155/2017/4046597
DO - 10.1155/2017/4046597
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AN - SCOPUS:85018854641
SN - 2090-004X
VL - 2017
JO - Journal of Ophthalmology
JF - Journal of Ophthalmology
M1 - 4046597
ER -