Characterization and localization of collagens synthesized by cultured muscle cells stimulated with collagen-inducing factor from embryonic brain extracts

D. Duksin, C. Kalcheim, Z. Vogel

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

A low molecular weight fraction (M(r) = 500-1500) was isolated by membrane and gel filtration from rat embryonic brain extract. This fraction was shown to stimulate collagen production in muscle cultures. Procollagen, intermediate collagenous proteins, and collagens of types I, III, and V were identified by analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and DEAE- and Cm-cellulose chromatography and by selective degradation by pepsin, bacterial collagenase, and cyanogen bromide. Immunofluorescent staining with antisera to collagen types I, III, IV, and V of muscle cultures treated with the collagen-inducing factor revealed a large increase in staining compared to control cultures and a distinct pattern of staining on the surface of the treated myotubes.

Original languageAmerican English
Pages (from-to)14585-14591
Number of pages7
JournalJournal of Biological Chemistry
Volume258
Issue number23
StatePublished - 1983
Externally publishedYes

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