TY - JOUR
T1 - Characterization of a branched-chain amino-acid aminotransferase from Schizosaccharomyces pombe
AU - Eden, Amir
AU - Benvenisty, Nissim
PY - 1998/1/30
Y1 - 1998/1/30
N2 - The Saccharomyces cerevisiae genes for the cytosolic and mitochondrial branched-chain amino-acid aminotransferases (BCAT) were isolated recently. These genes show significant homology to mammalian ECA39, originally isolated as a gene regulated by the c-myc oncogene. We now report the isolation of the Schizosaccharomyces pombe eca39/BCAT gene. The S. pombe protein shows 47-52% identity to other eukaryotic BCAT proteins isolated from S. cerevisiae, nematode, mouse and man. A genetic growth assay for BCAT activity was established using an S. cerevisiae strain disrupted in both BCAT isoenzymes. Consequently, the activity of the S. pombe BCAT was demonstrated by genetic and biochemical means. Possible applications of BCAT-encoding genes as selection markers in yeast transformation are proposed. The sequence has been deposited in the GenBank data library under Accession Number U88029.
AB - The Saccharomyces cerevisiae genes for the cytosolic and mitochondrial branched-chain amino-acid aminotransferases (BCAT) were isolated recently. These genes show significant homology to mammalian ECA39, originally isolated as a gene regulated by the c-myc oncogene. We now report the isolation of the Schizosaccharomyces pombe eca39/BCAT gene. The S. pombe protein shows 47-52% identity to other eukaryotic BCAT proteins isolated from S. cerevisiae, nematode, mouse and man. A genetic growth assay for BCAT activity was established using an S. cerevisiae strain disrupted in both BCAT isoenzymes. Consequently, the activity of the S. pombe BCAT was demonstrated by genetic and biochemical means. Possible applications of BCAT-encoding genes as selection markers in yeast transformation are proposed. The sequence has been deposited in the GenBank data library under Accession Number U88029.
KW - Aminotransferase
KW - Branched-chain amino acids
KW - Saccharomyces cerevisiae
KW - Schizosaccharomyces pombe
UR - http://www.scopus.com/inward/record.url?scp=0032579183&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0061(19980130)14:2<189::AID-YEA210>3.0.CO;2-V
DO - 10.1002/(SICI)1097-0061(19980130)14:2<189::AID-YEA210>3.0.CO;2-V
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C2 - 9483807
AN - SCOPUS:0032579183
SN - 0749-503X
VL - 14
SP - 189
EP - 194
JO - Yeast
JF - Yeast
IS - 2
ER -