Characterization of a Na+/H+ antiporter gene of Escherichia coli

E. B. Goldberg, T. Arbel, J. Chen, R. Karpel, G. A. Mackie, S. Schuldiner, E. Padan

Research output: Contribution to journalArticlepeer-review

196 Scopus citations

Abstract

A mutant of Escherichia coli with increased Na+/H+ antiport activity was isolated and found by other workers to harbor two mutations. The mutation that leads to increased Na+/H+ antiport (antup) has now been separated and mapped. antup maps in the vicinity of 0.5 min on the E. coli map, and the presence of this mutation alone in an isogenic pair raises antiport activity (V(max)) by ≃ 4-fold. We also characterized cells bearing plasmids containing fragments of a 15-kilobase-pair segment of DNA between carA and dnaJ. A wild-type gene located within 2 kilobase pairs counterclockwise of rpsT increases the Na+/H+ antiport activity when present in multiple copies.

Original languageEnglish
Pages (from-to)2615-2619
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number9
DOIs
StatePublished - 1987

Fingerprint

Dive into the research topics of 'Characterization of a Na+/H+ antiporter gene of Escherichia coli'. Together they form a unique fingerprint.

Cite this