TY - JOUR
T1 - Characterization of alginate-like exopolysaccharides isolated from aerobic granular sludge in pilot-plant
AU - Lin, Yuemei
AU - de Kreuk, Merle
AU - van Loosdrecht, M. C.M.
AU - Adin, Avner
PY - 2010/6
Y1 - 2010/6
N2 - To understand functional gel-forming exopolysaccharides in aerobic granular sludge, alginate-like exopolysaccharides were specifically extracted from aerobic granular sludge cultivated in a pilot plant treating municipal sewage. The exopolysaccharides were identified by the FAO/WHO alginate identification tests, characterized by biochemical assays, gelation with Ca2+, blocks fractionation, spectroscopic analysis as UV-visible, FT-IR and MALDI-TOF MS, and electrophoresis. The yield of extractable alginate-like exopolysaccharides was reached 160±4mg/g (VSS ratio). They resembled seaweed alginate in UV-visible and MALDI-TOF MS spectra, and distinguished from it in the reactions with acid ferric sulfate, phenol-sulfuric acid and Coomassie brilliant blue G250. Characterized by their high percentage of poly guluronic acid blocks (69.07±8.95%), the isolated exopolysaccharides were capable to form rigid, non-deformable gels in CaCl2. They were one of the dominant exopolysaccharides in aerobic granular sludge. We suggest that polymers play a significant role in providing aerobic granular sludge a highly hydrophobic, compact, strong and elastic structure.
AB - To understand functional gel-forming exopolysaccharides in aerobic granular sludge, alginate-like exopolysaccharides were specifically extracted from aerobic granular sludge cultivated in a pilot plant treating municipal sewage. The exopolysaccharides were identified by the FAO/WHO alginate identification tests, characterized by biochemical assays, gelation with Ca2+, blocks fractionation, spectroscopic analysis as UV-visible, FT-IR and MALDI-TOF MS, and electrophoresis. The yield of extractable alginate-like exopolysaccharides was reached 160±4mg/g (VSS ratio). They resembled seaweed alginate in UV-visible and MALDI-TOF MS spectra, and distinguished from it in the reactions with acid ferric sulfate, phenol-sulfuric acid and Coomassie brilliant blue G250. Characterized by their high percentage of poly guluronic acid blocks (69.07±8.95%), the isolated exopolysaccharides were capable to form rigid, non-deformable gels in CaCl2. They were one of the dominant exopolysaccharides in aerobic granular sludge. We suggest that polymers play a significant role in providing aerobic granular sludge a highly hydrophobic, compact, strong and elastic structure.
KW - Aerobic granular sludge
KW - Alginate
KW - Exopolysaccharides
KW - Extracellular polymeric substances
UR - http://www.scopus.com/inward/record.url?scp=77953020625&partnerID=8YFLogxK
U2 - 10.1016/j.watres.2010.03.019
DO - 10.1016/j.watres.2010.03.019
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 20417539
AN - SCOPUS:77953020625
SN - 0043-1354
VL - 44
SP - 3355
EP - 3364
JO - Water Research
JF - Water Research
IS - 11
ER -
