Characterization of Aspergillus niger B-1 RNase and its inhibitory effect of pollen germination and pollen tube growth in selected tree fruit

Levava Roiz; Uzi Ozeri; Raphael Goren; Oded Shoseyov

doi:10.21273/jashs.125.1.9

Characterization of Aspergillus niger B-1 RNase and its inhibitory effect of pollen germination and pollen tube growth in selected tree fruit

Levava Roiz^*, Uzi Ozeri, Raphael Goren, Oded Shoseyov

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Aspergillus niger B-1 (CMI CC 324626) extracellular RNase (RNase B1) was purified to homogeneity. It was found to contain two isoforms of 32- and 40-kDa glucoproteins, sharing a 29-kDa protein moiety. Optimal RNase activity was observed at 60°C and pH 3.5. In 'Almog' peach [Prunus persica (L.) Batsch (Peach Group) 'Almog'] and 'Murcott' tangerine (Citrus reticulata Blanco 'Murcott') the enzyme inhibited pollen germination and pollen tube growth in vitro as well as in vivo. In field experiments, spray application of the RNase caused a reduction in 'Fantasia' nectarine [Prunus persica (L.) Batsch (Nectarine Group) 'Fantasia'] fruit set and interfered with embryo development. The biological effect of the RNase may be of horticultural value, due to its potential to control fertilization.

Original language	American English
Pages (from-to)	9-14
Number of pages	6
Journal	Journal of the American Society for Horticultural Science
Volume	125
Issue number	1
DOIs	https://doi.org/10.21273/jashs.125.1.9
State	Published - Jan 2000

Keywords

Citrus reticulata
Peach
Pollen tube growth
Prunus persica (Peach Group)
RNAse B1
Tangerine

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title = "Characterization of Aspergillus niger B-1 RNase and its inhibitory effect of pollen germination and pollen tube growth in selected tree fruit",

abstract = "Aspergillus niger B-1 (CMI CC 324626) extracellular RNase (RNase B1) was purified to homogeneity. It was found to contain two isoforms of 32- and 40-kDa glucoproteins, sharing a 29-kDa protein moiety. Optimal RNase activity was observed at 60°C and pH 3.5. In 'Almog' peach [Prunus persica (L.) Batsch (Peach Group) 'Almog'] and 'Murcott' tangerine (Citrus reticulata Blanco 'Murcott') the enzyme inhibited pollen germination and pollen tube growth in vitro as well as in vivo. In field experiments, spray application of the RNase caused a reduction in 'Fantasia' nectarine [Prunus persica (L.) Batsch (Nectarine Group) 'Fantasia'] fruit set and interfered with embryo development. The biological effect of the RNase may be of horticultural value, due to its potential to control fertilization.",

keywords = "Citrus reticulata, Peach, Pollen tube growth, Prunus persica (Peach Group), RNAse B1, Tangerine",

author = "Levava Roiz and Uzi Ozeri and Raphael Goren and Oded Shoseyov",

year = "2000",

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AU - Roiz, Levava

AU - Ozeri, Uzi

AU - Goren, Raphael

AU - Shoseyov, Oded

PY - 2000/1

Y1 - 2000/1

N2 - Aspergillus niger B-1 (CMI CC 324626) extracellular RNase (RNase B1) was purified to homogeneity. It was found to contain two isoforms of 32- and 40-kDa glucoproteins, sharing a 29-kDa protein moiety. Optimal RNase activity was observed at 60°C and pH 3.5. In 'Almog' peach [Prunus persica (L.) Batsch (Peach Group) 'Almog'] and 'Murcott' tangerine (Citrus reticulata Blanco 'Murcott') the enzyme inhibited pollen germination and pollen tube growth in vitro as well as in vivo. In field experiments, spray application of the RNase caused a reduction in 'Fantasia' nectarine [Prunus persica (L.) Batsch (Nectarine Group) 'Fantasia'] fruit set and interfered with embryo development. The biological effect of the RNase may be of horticultural value, due to its potential to control fertilization.

AB - Aspergillus niger B-1 (CMI CC 324626) extracellular RNase (RNase B1) was purified to homogeneity. It was found to contain two isoforms of 32- and 40-kDa glucoproteins, sharing a 29-kDa protein moiety. Optimal RNase activity was observed at 60°C and pH 3.5. In 'Almog' peach [Prunus persica (L.) Batsch (Peach Group) 'Almog'] and 'Murcott' tangerine (Citrus reticulata Blanco 'Murcott') the enzyme inhibited pollen germination and pollen tube growth in vitro as well as in vivo. In field experiments, spray application of the RNase caused a reduction in 'Fantasia' nectarine [Prunus persica (L.) Batsch (Nectarine Group) 'Fantasia'] fruit set and interfered with embryo development. The biological effect of the RNase may be of horticultural value, due to its potential to control fertilization.

KW - Citrus reticulata

KW - Peach

KW - Pollen tube growth

KW - Prunus persica (Peach Group)

KW - RNAse B1

KW - Tangerine

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Characterization of Aspergillus niger B-1 RNase and its inhibitory effect of pollen germination and pollen tube growth in selected tree fruit

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