TY - JOUR
T1 - Characterization of catechol derivative removal by lignin peroxidase in aqueous mixture
AU - Cohen, Shaul
AU - Belinky, Paula A.
AU - Hadar, Yitzhak
AU - Dosoretz, Carlos G.
PY - 2009/4
Y1 - 2009/4
N2 - The use of lignin peroxidase (LIP) as an alternative method for the removal of four catechols (1,2-dihydroxybenzene): catechol (CAT), 4-chlorocatechol (4-CC), 4,5-dichlorocatechol (4,5-DCC) and 4-methylcatechol (4-MC) typical pollutants in wastewater derived from oil and paper industries, was evaluated. The removal of 2 mM catecholic substrates by 1 μM LIP after 1 h was in the following order: 4,5-DCC (95%) > 4-CC(90%) > CAT(55%) > 4-MC(43%). Except for 4-MC, all reactions were accompanied by the formation of insoluble products, leading to LIP precipitation. LIP was exposed to soluble or insoluble product-dependent inactivation, depending on the substrates tested, immediately at the start of the reactions. Despite immediate enzyme inactivation, removal of catecholic substrates continued, resulting in oligomeric product formation. Major oxidation products analyzed were compatible with dimeric, trimeric and tetrameric structures. Ether linkages and a benzoquinone structure were detected in two purified oligochlorocatechols. Catechol derivatives removal initiated by LIP, seems to be different for each catecholic substrate in terms of substrate consumption and transformation, and of enzyme activity.
AB - The use of lignin peroxidase (LIP) as an alternative method for the removal of four catechols (1,2-dihydroxybenzene): catechol (CAT), 4-chlorocatechol (4-CC), 4,5-dichlorocatechol (4,5-DCC) and 4-methylcatechol (4-MC) typical pollutants in wastewater derived from oil and paper industries, was evaluated. The removal of 2 mM catecholic substrates by 1 μM LIP after 1 h was in the following order: 4,5-DCC (95%) > 4-CC(90%) > CAT(55%) > 4-MC(43%). Except for 4-MC, all reactions were accompanied by the formation of insoluble products, leading to LIP precipitation. LIP was exposed to soluble or insoluble product-dependent inactivation, depending on the substrates tested, immediately at the start of the reactions. Despite immediate enzyme inactivation, removal of catecholic substrates continued, resulting in oligomeric product formation. Major oxidation products analyzed were compatible with dimeric, trimeric and tetrameric structures. Ether linkages and a benzoquinone structure were detected in two purified oligochlorocatechols. Catechol derivatives removal initiated by LIP, seems to be different for each catecholic substrate in terms of substrate consumption and transformation, and of enzyme activity.
KW - Catechol removal
KW - Lignin peroxidase
KW - Phenol removal
KW - Product-dependent inactivation
KW - Wastewater treatment
UR - http://www.scopus.com/inward/record.url?scp=58149191210&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2008.11.007
DO - 10.1016/j.biortech.2008.11.007
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 19097884
AN - SCOPUS:58149191210
SN - 0960-8524
VL - 100
SP - 2247
EP - 2253
JO - Bioresource Technology
JF - Bioresource Technology
IS - 7
ER -
