Characterization of Cytoplasmic DNA of Mouse‐Myeloma Cells

Adina SOLAGE*, Reuven LASKOV

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Cytoplasmic DNA from mouse myeloma cells comprised between 1 % and 2 % of the total cellular DNA. Detergent‐prepared cytoplasmic lysate consisted mainly of 8‐S and 22‐S species. While these DNA species were present in the 13000×g pellet of the detergent‐prepared cytoplasmic lysate. only the light DNA species was present in the 13000×g supernatant fraction. In neutral CsCI gradients the DNA of both cytoplasmic fractions had a buoyant density of 1700 g/cm3, which is identical to that of nuclear DNA. The similarity between the cytoplasmic and nuclear DNA was also demonstrated by analysis on alkaline CsCl gradients. A small proportion of closed‐circular DNA, presumably of mitochondrial origin, was demonstrated only in cytoplasmic fraction obtained from mechanically disrupted cells and not in detergent‐prepared cytoplasmic lysate. It was found that poly(A)‐containing mRNA and 28‐S ribosomal RNA hybridized to about the same extent to the cytoplasmic DNA as compared to nuclear DNA. The results indicate that most of the cytoplasmic DNA in myeloma cells is similar to nuclear DNA and does not consist of mitochondrial DNA.

Original languageEnglish
Pages (from-to)23-33
Number of pages11
JournalEuropean Journal of Biochemistry
Volume60
Issue number1
DOIs
StatePublished - Dec 1975

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