TY - JOUR
T1 - Characterization of Cytoplasmic DNA of Mouse‐Myeloma Cells
AU - SOLAGE, Adina
AU - LASKOV, Reuven
PY - 1975/12
Y1 - 1975/12
N2 - Cytoplasmic DNA from mouse myeloma cells comprised between 1 % and 2 % of the total cellular DNA. Detergent‐prepared cytoplasmic lysate consisted mainly of 8‐S and 22‐S species. While these DNA species were present in the 13000×g pellet of the detergent‐prepared cytoplasmic lysate. only the light DNA species was present in the 13000×g supernatant fraction. In neutral CsCI gradients the DNA of both cytoplasmic fractions had a buoyant density of 1700 g/cm3, which is identical to that of nuclear DNA. The similarity between the cytoplasmic and nuclear DNA was also demonstrated by analysis on alkaline CsCl gradients. A small proportion of closed‐circular DNA, presumably of mitochondrial origin, was demonstrated only in cytoplasmic fraction obtained from mechanically disrupted cells and not in detergent‐prepared cytoplasmic lysate. It was found that poly(A)‐containing mRNA and 28‐S ribosomal RNA hybridized to about the same extent to the cytoplasmic DNA as compared to nuclear DNA. The results indicate that most of the cytoplasmic DNA in myeloma cells is similar to nuclear DNA and does not consist of mitochondrial DNA.
AB - Cytoplasmic DNA from mouse myeloma cells comprised between 1 % and 2 % of the total cellular DNA. Detergent‐prepared cytoplasmic lysate consisted mainly of 8‐S and 22‐S species. While these DNA species were present in the 13000×g pellet of the detergent‐prepared cytoplasmic lysate. only the light DNA species was present in the 13000×g supernatant fraction. In neutral CsCI gradients the DNA of both cytoplasmic fractions had a buoyant density of 1700 g/cm3, which is identical to that of nuclear DNA. The similarity between the cytoplasmic and nuclear DNA was also demonstrated by analysis on alkaline CsCl gradients. A small proportion of closed‐circular DNA, presumably of mitochondrial origin, was demonstrated only in cytoplasmic fraction obtained from mechanically disrupted cells and not in detergent‐prepared cytoplasmic lysate. It was found that poly(A)‐containing mRNA and 28‐S ribosomal RNA hybridized to about the same extent to the cytoplasmic DNA as compared to nuclear DNA. The results indicate that most of the cytoplasmic DNA in myeloma cells is similar to nuclear DNA and does not consist of mitochondrial DNA.
UR - http://www.scopus.com/inward/record.url?scp=0016754199&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1975.tb20971.x
DO - 10.1111/j.1432-1033.1975.tb20971.x
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C2 - 1204639
AN - SCOPUS:0016754199
SN - 0014-2956
VL - 60
SP - 23
EP - 33
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 1
ER -