Characterization of extremely halophilic Archaea isolated from the Ayvalik Saltern, Turkey

Rahel Elevi, Parvaneh Assa*, Meral Birbir, Ayse Ogan, Aharon Oren

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Seven extremely halophilic strains were isolated from the Ayvalik Saltern in the north-eastern part of Turkey. Chemical analyses of the brine and salt samples were performed to measure their salt content, hardness and pH. Isolated strains were tested for their antibiotic sensitivities; cell and colony morphologies; hydrolysis of casein, starch, gelatin, Tween 20 and Tween 80; and oxidase and catalase activity. All strains were found to belong to the domain Archaea. Characterization of polar lipids by thin layer chromatography indicated that all isolates contained phytanyl diether derivatives of phosphatidylglycerol (PG), the methyl ester of phosphatidyl glycerophosphate (PGP-Me), and phosphatidylglycerosulphate (PGS). Four isolates had triglycosyl diether (TGD-2) as glycolipid, and the other three contained a sulphated diglycosyl diether instead. All isolates were examined for the presence of plasmids by agarose gel electrophoresis. Four strains were found to harbour plasmids ranging in size from 13.8 to 15.3 kbp. Correlation between the protein profiles in SDS-PAGE and the phenotypic properties of the strains was poor. The data presented here provide the first published account of the microbiota of the Ayvalik saltern, which provides a large part of the salt produced in Turkey.

Original languageAmerican English
Pages (from-to)719-725
Number of pages7
JournalWorld Journal of Microbiology and Biotechnology
Volume20
Issue number7
DOIs
StatePublished - Oct 2004

Bibliographical note

Funding Information:
We would like to thank Dr Baris Calli and Bulent Mertoglu for the chemical analyses of brine and salt samples, and Prof Dr Engin Ozatay for the use of his laboratory facilities. We thank the Research Foundation of Marmara University for a grant. Rahel Elevi was

Funding Information:
supported by a scholarship from the Israel Ministry of Foreign Affairs, under the Bilateral Cultural and Scientific Agreement with Turkey.

Keywords

  • Archaea
  • Exoenzymes
  • Extremely halophilic Archaea
  • Salterns
  • Turkey

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