TY - JOUR
T1 - Characterization of gastrulation-stage progenitor cells and their inhibitory crosstalk in human embryoid bodies
AU - Kopper, Oded
AU - Giladi, Odil
AU - Golan-Lev, Tamar
AU - Benvenisty, Nissim
PY - 2010/1
Y1 - 2010/1
N2 - Human embryoid bodies (HEBs) are cell aggregates that are produced during the course of embryonic stem cell differentiation in suspension. Mature HEBs have been shown to contain derivatives of the three embryonic germ layers. In this study, using a combination of laser capture microscopy followed by DNA microarray analysis and cell sorting, we demonstrate that early HEBs are composed of three major cell populations. These cell populations can be defined by the expression of specific cell markers, namely: (i) OCT4+, REX1-; (ii) NCAD+, OCT4-; and (iii) EPOR +, OCT4-. By analyzing gene expression in embryonic tissues, these cell populations could respectively be assigned to the embryonic ectoderm, mesendoderm, and extraembryonic endoderm lineages. We show that the extraembryonic endoderm, which selectively expresses platelet-derived growth factor B (PDGF-B), negatively affects the mesendoderm lineage, which selectively expresses the receptor PDGFRA. Our analysis suggests that early HEBs are spatially patterned and that cell differentiation is governed by interactions between the different cell types.
AB - Human embryoid bodies (HEBs) are cell aggregates that are produced during the course of embryonic stem cell differentiation in suspension. Mature HEBs have been shown to contain derivatives of the three embryonic germ layers. In this study, using a combination of laser capture microscopy followed by DNA microarray analysis and cell sorting, we demonstrate that early HEBs are composed of three major cell populations. These cell populations can be defined by the expression of specific cell markers, namely: (i) OCT4+, REX1-; (ii) NCAD+, OCT4-; and (iii) EPOR +, OCT4-. By analyzing gene expression in embryonic tissues, these cell populations could respectively be assigned to the embryonic ectoderm, mesendoderm, and extraembryonic endoderm lineages. We show that the extraembryonic endoderm, which selectively expresses platelet-derived growth factor B (PDGF-B), negatively affects the mesendoderm lineage, which selectively expresses the receptor PDGFRA. Our analysis suggests that early HEBs are spatially patterned and that cell differentiation is governed by interactions between the different cell types.
KW - Embryogenesis
KW - Human embryoid bodies
KW - Human embryonic stem cells
KW - Progenitor cells
UR - http://www.scopus.com/inward/record.url?scp=75349090584&partnerID=8YFLogxK
U2 - 10.1002/stem.260
DO - 10.1002/stem.260
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C2 - 19921748
AN - SCOPUS:75349090584
SN - 1066-5099
VL - 28
SP - 75
EP - 83
JO - Stem Cells
JF - Stem Cells
IS - 1
ER -