Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Oluseun Adewumi; Behrouz Aflatoonian; Lars Ahrlund-Richter; Michal Amit; Peter W. Andrews; Gemma Beighton; Paul A. Bello; Nissim Benvenisty; Lorraine S. Berry; Simon Bevan; Barak Blum; Justin Brooking; Kevin G. Chen; Andre B.H. Choo; Gary A. Churchill; Marie Corbel; Ivan Damjanov; Jon S. Draper; Petr Dvorak; Katarina Emanuelsson; Roland A. Fleck; Angela Ford; Karin Gertow; Marina Gertsenstein; Paul J. Gokhale; Rebecca S. Hamilton; Ales Hampl; Lyn E. Healy; Outi Hovatta; Johan Hyllner; Marta P. Imreh; Joseph Itskovitz-Eldor; Jamie Jackson; Jacqueline L. Johnson; Mark Jones; Kehkooi Kee; Benjamin L. King; Barbara B. Knowles; Majlinda Lako; Franck Lebrin; Barbara S. Mallon; Daisy Manning; Yoav Mayshar; Ronald D.G. McKay; Anna E. Michalska; Milla Mikkola; Masha Mileikovsky; Stephen L. Minger; Harry D. Moore; Christine L. Mummery; Andras Nagy; Norio Nakatsuji; Carmel M. O'Brien; Steve K.W. Oh; Cia Olsson; Timo Otonkoski; Kye Yoon Park; Robert Passier; Hema Patel; Minal Patel; Roger Pedersen; Martin F. Pera; Marian S. Piekarczyk; Renee A.Reijo Pera; Benjamin E. Reubinoff; Allan J. Robins; Janet Rossant; Peter Rugg-Gunn; Thomas C. Schulz; Henrik Semb; Eric S. Sherrer; Henrike Siemen; Glyn N. Stacey; Miodrag Stojkovic; Hirofumi Suemori; Jin Szatkiewicz; Tikva Turetsky; Timo Tuuri; Steineke Van Den Brink; Kristina Vintersten; Sanna Vuoristo; Dorien Ward; Thomas A. Weaver; Lesley A. Young; Weidong Zhang

doi:10.1038/nbt1318

Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Oluseun Adewumi, Behrouz Aflatoonian, Lars Ahrlund-Richter, Michal Amit, Peter W. Andrews^*, Gemma Beighton, Paul A. Bello, Nissim Benvenisty, Lorraine S. Berry, Simon Bevan, Barak Blum, Justin Brooking, Kevin G. Chen, Andre B.H. Choo, Gary A. Churchill, Marie Corbel, Ivan Damjanov, Jon S. Draper, Petr Dvorak, Katarina EmanuelssonRoland A. Fleck, Angela Ford, Karin Gertow, Marina Gertsenstein, Paul J. Gokhale, Rebecca S. Hamilton, Ales Hampl, Lyn E. Healy, Outi Hovatta, Johan Hyllner, Marta P. Imreh, Joseph Itskovitz-Eldor, Jamie Jackson, Jacqueline L. Johnson, Mark Jones, Kehkooi Kee, Benjamin L. King, Barbara B. Knowles, Majlinda Lako, Franck Lebrin, Barbara S. Mallon, Daisy Manning, Yoav Mayshar, Ronald D.G. McKay, Anna E. Michalska, Milla Mikkola, Masha Mileikovsky, Stephen L. Minger, Harry D. Moore, Christine L. Mummery, Andras Nagy, Norio Nakatsuji, Carmel M. O'Brien, Steve K.W. Oh, Cia Olsson, Timo Otonkoski, Kye Yoon Park, Robert Passier, Hema Patel, Minal Patel, Roger Pedersen, Martin F. Pera, Marian S. Piekarczyk, Renee A.Reijo Pera, Benjamin E. Reubinoff, Allan J. Robins, Janet Rossant, Peter Rugg-Gunn, Thomas C. Schulz, Henrik Semb, Eric S. Sherrer, Henrike Siemen, Glyn N. Stacey, Miodrag Stojkovic, Hirofumi Suemori, Jin Szatkiewicz, Tikva Turetsky, Timo Tuuri, Steineke Van Den Brink, Kristina Vintersten, Sanna Vuoristo, Dorien Ward, Thomas A. Weaver, Lesley A. Young, Weidong Zhang

^*Corresponding author for this work

Department of Genetics

Research output: Contribution to journal › Article › peer-review

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Abstract

The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

Original language	English
Pages (from-to)	803-816
Number of pages	14
Journal	Nature Biotechnology
Volume	25
Issue number	7
DOIs	https://doi.org/10.1038/nbt1318
State	Published - Jul 2007

Bibliographical note

Funding Information:
The International Stem Cell Initiative was sponsored and supported by the International Stem Cell Forum. Additional support from individual participants, and for elements of the project, was also provided by: A*STAR; Academy of Sciences of the Czech Republic; The Academy of Finland; Applied Biosystems; Axordia Ltd.; Bayer; Biotechnology and Biological Sciences Research Council, Centre for Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic; Chemicon now part of Millipore; Dutch Platform for Tissue Engineering; The Ellison Medical Foundation; Engineering and Physical Sciences Research Council; Embryonic Stem Cell International; GE Healthcare; The Helsinki University Central Hospital Research; Invitrogen Corporation; The Juvenile Diabetes Research Foundation; The Medical Research Council; The Ministry of Education, Culture, Sports, Science, & Technology of Japan; The National Institute for Biological Standards & Control; National Institutes of Health grants 9R24RR021314-04, 5R24RR018405, 5R24RR017498-04; One North East Regional Developmental Agency; The Shelby Cullom Davis Foundation; Stem Cell Network Canada; Stem Cells in Development and Disease; Swedish Research Council. We would like to thank the following for their invaluable assistance with the project: Marga van Rooijen, Hubrecht Laboratory; Christine Pigott, University of Sheffield; Ludmila Ruban, University

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Adewumi, O., Aflatoonian, B., Ahrlund-Richter, L., Amit, M., Andrews, P. W., Beighton, G., Bello, P. A., Benvenisty, N., Berry, L. S., Bevan, S., Blum, B., Brooking, J., Chen, K. G., Choo, A. B. H., Churchill, G. A., Corbel, M., Damjanov, I., Draper, J. S., Dvorak, P., ... Zhang, W. (2007). Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nature Biotechnology, 25(7), 803-816. https://doi.org/10.1038/nbt1318

@article{9314de668e9d43cab01e941c0aa7f365,

title = "Characterization of human embryonic stem cell lines by the International Stem Cell Initiative",

abstract = "The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.",

author = "Oluseun Adewumi and Behrouz Aflatoonian and Lars Ahrlund-Richter and Michal Amit and Andrews, {Peter W.} and Gemma Beighton and Bello, {Paul A.} and Nissim Benvenisty and Berry, {Lorraine S.} and Simon Bevan and Barak Blum and Justin Brooking and Chen, {Kevin G.} and Choo, {Andre B.H.} and Churchill, {Gary A.} and Marie Corbel and Ivan Damjanov and Draper, {Jon S.} and Petr Dvorak and Katarina Emanuelsson and Fleck, {Roland A.} and Angela Ford and Karin Gertow and Marina Gertsenstein and Gokhale, {Paul J.} and Hamilton, {Rebecca S.} and Ales Hampl and Healy, {Lyn E.} and Outi Hovatta and Johan Hyllner and Imreh, {Marta P.} and Joseph Itskovitz-Eldor and Jamie Jackson and Johnson, {Jacqueline L.} and Mark Jones and Kehkooi Kee and King, {Benjamin L.} and Knowles, {Barbara B.} and Majlinda Lako and Franck Lebrin and Mallon, {Barbara S.} and Daisy Manning and Yoav Mayshar and McKay, {Ronald D.G.} and Michalska, {Anna E.} and Milla Mikkola and Masha Mileikovsky and Minger, {Stephen L.} and Moore, {Harry D.} and Mummery, {Christine L.} and Andras Nagy and Norio Nakatsuji and O'Brien, {Carmel M.} and Oh, {Steve K.W.} and Cia Olsson and Timo Otonkoski and Park, {Kye Yoon} and Robert Passier and Hema Patel and Minal Patel and Roger Pedersen and Pera, {Martin F.} and Piekarczyk, {Marian S.} and Pera, {Renee A.Reijo} and Reubinoff, {Benjamin E.} and Robins, {Allan J.} and Janet Rossant and Peter Rugg-Gunn and Schulz, {Thomas C.} and Henrik Semb and Sherrer, {Eric S.} and Henrike Siemen and Stacey, {Glyn N.} and Miodrag Stojkovic and Hirofumi Suemori and Jin Szatkiewicz and Tikva Turetsky and Timo Tuuri and {Van Den Brink}, Steineke and Kristina Vintersten and Sanna Vuoristo and Dorien Ward and Weaver, {Thomas A.} and Young, {Lesley A.} and Weidong Zhang",

note = "Funding Information: The International Stem Cell Initiative was sponsored and supported by the International Stem Cell Forum. Additional support from individual participants, and for elements of the project, was also provided by: A*STAR; Academy of Sciences of the Czech Republic; The Academy of Finland; Applied Biosystems; Axordia Ltd.; Bayer; Biotechnology and Biological Sciences Research Council, Centre for Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic; Chemicon now part of Millipore; Dutch Platform for Tissue Engineering; The Ellison Medical Foundation; Engineering and Physical Sciences Research Council; Embryonic Stem Cell International; GE Healthcare; The Helsinki University Central Hospital Research; Invitrogen Corporation; The Juvenile Diabetes Research Foundation; The Medical Research Council; The Ministry of Education, Culture, Sports, Science, & Technology of Japan; The National Institute for Biological Standards & Control; National Institutes of Health grants 9R24RR021314-04, 5R24RR018405, 5R24RR017498-04; One North East Regional Developmental Agency; The Shelby Cullom Davis Foundation; Stem Cell Network Canada; Stem Cells in Development and Disease; Swedish Research Council. We would like to thank the following for their invaluable assistance with the project: Marga van Rooijen, Hubrecht Laboratory; Christine Pigott, University of Sheffield; Ludmila Ruban, University",

year = "2007",

month = jul,

doi = "10.1038/nbt1318",

language = "אנגלית",

volume = "25",

pages = "803--816",

journal = "Nature Biotechnology",

issn = "1087-0156",

publisher = "Nature Research",

number = "7",

}

Adewumi, O, Aflatoonian, B, Ahrlund-Richter, L, Amit, M, Andrews, PW, Beighton, G, Bello, PA, Benvenisty, N, Berry, LS, Bevan, S, Blum, B, Brooking, J, Chen, KG, Choo, ABH, Churchill, GA, Corbel, M, Damjanov, I, Draper, JS, Dvorak, P, Emanuelsson, K, Fleck, RA, Ford, A, Gertow, K, Gertsenstein, M, Gokhale, PJ, Hamilton, RS, Hampl, A, Healy, LE, Hovatta, O, Hyllner, J, Imreh, MP, Itskovitz-Eldor, J, Jackson, J, Johnson, JL, Jones, M, Kee, K, King, BL, Knowles, BB, Lako, M, Lebrin, F, Mallon, BS, Manning, D, Mayshar, Y, McKay, RDG, Michalska, AE, Mikkola, M, Mileikovsky, M, Minger, SL, Moore, HD, Mummery, CL, Nagy, A, Nakatsuji, N, O'Brien, CM, Oh, SKW, Olsson, C, Otonkoski, T, Park, KY, Passier, R, Patel, H, Patel, M, Pedersen, R, Pera, MF, Piekarczyk, MS, Pera, RAR, Reubinoff, BE, Robins, AJ, Rossant, J, Rugg-Gunn, P, Schulz, TC, Semb, H, Sherrer, ES, Siemen, H, Stacey, GN, Stojkovic, M, Suemori, H, Szatkiewicz, J, Turetsky, T, Tuuri, T, Van Den Brink, S, Vintersten, K, Vuoristo, S, Ward, D, Weaver, TA, Young, LA & Zhang, W 2007, 'Characterization of human embryonic stem cell lines by the International Stem Cell Initiative', Nature Biotechnology, vol. 25, no. 7, pp. 803-816. https://doi.org/10.1038/nbt1318

TY - JOUR

T1 - Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

AU - Adewumi, Oluseun

AU - Aflatoonian, Behrouz

AU - Ahrlund-Richter, Lars

AU - Amit, Michal

AU - Andrews, Peter W.

AU - Beighton, Gemma

AU - Bello, Paul A.

AU - Benvenisty, Nissim

AU - Berry, Lorraine S.

AU - Bevan, Simon

AU - Blum, Barak

AU - Brooking, Justin

AU - Chen, Kevin G.

AU - Choo, Andre B.H.

AU - Churchill, Gary A.

AU - Corbel, Marie

AU - Damjanov, Ivan

AU - Draper, Jon S.

AU - Dvorak, Petr

AU - Emanuelsson, Katarina

AU - Fleck, Roland A.

AU - Ford, Angela

AU - Gertow, Karin

AU - Gertsenstein, Marina

AU - Gokhale, Paul J.

AU - Hamilton, Rebecca S.

AU - Hampl, Ales

AU - Healy, Lyn E.

AU - Hovatta, Outi

AU - Hyllner, Johan

AU - Imreh, Marta P.

AU - Itskovitz-Eldor, Joseph

AU - Jackson, Jamie

AU - Johnson, Jacqueline L.

AU - Jones, Mark

AU - Kee, Kehkooi

AU - King, Benjamin L.

AU - Knowles, Barbara B.

AU - Lako, Majlinda

AU - Lebrin, Franck

AU - Mallon, Barbara S.

AU - Manning, Daisy

AU - Mayshar, Yoav

AU - McKay, Ronald D.G.

AU - Michalska, Anna E.

AU - Mikkola, Milla

AU - Mileikovsky, Masha

AU - Minger, Stephen L.

AU - Moore, Harry D.

AU - Mummery, Christine L.

AU - Nagy, Andras

AU - Nakatsuji, Norio

AU - O'Brien, Carmel M.

AU - Oh, Steve K.W.

AU - Olsson, Cia

AU - Otonkoski, Timo

AU - Park, Kye Yoon

AU - Passier, Robert

AU - Patel, Hema

AU - Patel, Minal

AU - Pedersen, Roger

AU - Pera, Martin F.

AU - Piekarczyk, Marian S.

AU - Pera, Renee A.Reijo

AU - Reubinoff, Benjamin E.

AU - Robins, Allan J.

AU - Rossant, Janet

AU - Rugg-Gunn, Peter

AU - Schulz, Thomas C.

AU - Semb, Henrik

AU - Sherrer, Eric S.

AU - Siemen, Henrike

AU - Stacey, Glyn N.

AU - Stojkovic, Miodrag

AU - Suemori, Hirofumi

AU - Szatkiewicz, Jin

AU - Turetsky, Tikva

AU - Tuuri, Timo

AU - Van Den Brink, Steineke

AU - Vintersten, Kristina

AU - Vuoristo, Sanna

AU - Ward, Dorien

AU - Weaver, Thomas A.

AU - Young, Lesley A.

AU - Zhang, Weidong

N1 - Funding Information: The International Stem Cell Initiative was sponsored and supported by the International Stem Cell Forum. Additional support from individual participants, and for elements of the project, was also provided by: A*STAR; Academy of Sciences of the Czech Republic; The Academy of Finland; Applied Biosystems; Axordia Ltd.; Bayer; Biotechnology and Biological Sciences Research Council, Centre for Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic; Chemicon now part of Millipore; Dutch Platform for Tissue Engineering; The Ellison Medical Foundation; Engineering and Physical Sciences Research Council; Embryonic Stem Cell International; GE Healthcare; The Helsinki University Central Hospital Research; Invitrogen Corporation; The Juvenile Diabetes Research Foundation; The Medical Research Council; The Ministry of Education, Culture, Sports, Science, & Technology of Japan; The National Institute for Biological Standards & Control; National Institutes of Health grants 9R24RR021314-04, 5R24RR018405, 5R24RR017498-04; One North East Regional Developmental Agency; The Shelby Cullom Davis Foundation; Stem Cell Network Canada; Stem Cells in Development and Disease; Swedish Research Council. We would like to thank the following for their invaluable assistance with the project: Marga van Rooijen, Hubrecht Laboratory; Christine Pigott, University of Sheffield; Ludmila Ruban, University

PY - 2007/7

Y1 - 2007/7

N2 - The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

AB - The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

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U2 - 10.1038/nbt1318

DO - 10.1038/nbt1318

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C2 - 17572666

AN - SCOPUS:34447295350

SN - 1087-0156

VL - 25

SP - 803

EP - 816

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 7

ER -

Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

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