Characterization of lactogen receptors in lactogenic hormone-dependent and independent NB2 lymphoma cell lines

A. Ashkenazi, R. Cohen, A. Gertler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A comparative study of lactogen receptors in lactogenic hormone-dependent (Nb2-11C) and independent (Nb2-SP) rat lymphoma cell lines revealed no significant differences in their binding and structural characteristics. The affinity for human growth hormone (hGH) and ovine prolactin is identical (Kd) = 1.3 × 10-10M). Exposure to hGH results in a rapid receptor down-regulation. This process is faster in Nb2-SP cells, however, similar receptor levels are reached within 3 h. Affinity labeling revealed 2 major binding species (Mr 75 000 and 29000) in both cell lines. These observations suggest that the spontaneous proliferation of Nb2-SP cells probably results from an aberration in some post-receptoral event of the mitogenic pathway.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalFEBS Letters
Volume210
Issue number1
DOIs
StatePublished - 1 Jan 1987

Keywords

  • (Nb2 lymphoma cell)
  • Human growth hormone
  • Lactogen receptor
  • Prolactin

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