TY - JOUR
T1 - Characterization of liposomes and other lipid assemblies by multiprobe fluorescence polarization
AU - Borenstain, Vered
AU - Barenholz, Yechezkel
PY - 1993/9
Y1 - 1993/9
N2 - This minireview describes the use of fluorescence polarization to characterize the dynamics and organization of biological membranes, lipoproteins, liposomes and other lipid-based assemblies. The advantages, disadvantages and limitations of this method are discussed. There is special emphasis on the multiprobe approach, which can monitor various parts of the lipid membrane or assembly. The fluorescence polarization, anisotropy and the total fluorescence intensity are defined and their graphic presentation described. A user guide for the actual measurements is given. It describes how to apply fluorescence polarization, especially how to select the proper extrinsic probe and to optimize the measurement regarding theoretical and practical issues, the determination of a fluorescent probe location in the assembly, how to introduce the probe to the lipid assembly, what is the ideal ratio of the probe to the lipid, dealing with the spectrofluorimeter problems and how to overcome disturbances introduced by light scattering. It is shown here, that even though there are disadvantages in using fluorescence polarization, this approach provides a highly sensitive and simple means for assessing the subtle and more pronounced changes in the structure of lipid assemblies.
AB - This minireview describes the use of fluorescence polarization to characterize the dynamics and organization of biological membranes, lipoproteins, liposomes and other lipid-based assemblies. The advantages, disadvantages and limitations of this method are discussed. There is special emphasis on the multiprobe approach, which can monitor various parts of the lipid membrane or assembly. The fluorescence polarization, anisotropy and the total fluorescence intensity are defined and their graphic presentation described. A user guide for the actual measurements is given. It describes how to apply fluorescence polarization, especially how to select the proper extrinsic probe and to optimize the measurement regarding theoretical and practical issues, the determination of a fluorescent probe location in the assembly, how to introduce the probe to the lipid assembly, what is the ideal ratio of the probe to the lipid, dealing with the spectrofluorimeter problems and how to overcome disturbances introduced by light scattering. It is shown here, that even though there are disadvantages in using fluorescence polarization, this approach provides a highly sensitive and simple means for assessing the subtle and more pronounced changes in the structure of lipid assemblies.
KW - fluorescent anisotropy
KW - fluorescent polarization
KW - fluorescent probes
KW - lipid assembly
KW - liposome
KW - liposome quality control
KW - multiprobe
UR - http://www.scopus.com/inward/record.url?scp=0027501420&partnerID=8YFLogxK
U2 - 10.1016/0009-3084(93)90061-7
DO - 10.1016/0009-3084(93)90061-7
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C2 - 8242830
AN - SCOPUS:0027501420
SN - 0009-3084
VL - 64
SP - 117
EP - 127
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
IS - 1-3
ER -