Characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria

C. C. Neto; K. A. Penndorf; M. Feldman; S. Meron-Sudai; Z. Zakay-Rones; D. Steinberg; M. Fridman; Y. Kashman; I. Ginsburg; I. Ofek; E. I. Weiss

doi:10.1039/c7fo00109f

Characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria

C. C. Neto^*, K. A. Penndorf, M. Feldman, S. Meron-Sudai, Z. Zakay-Rones, D. Steinberg, M. Fridman, Y. Kashman, I. Ginsburg, I. Ofek, E. I. Weiss

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

An extract prepared from cranberry juice by dialysis known as nondialyzable material (NDM) has been shown previously to possess anti-adhesion activity toward microbial species including oral bacteria, uropathogenic Escherichia coli and Helicobacter pylori. Bioassay-guided fractionation of cranberry NDM was therefore undertaken to identify the anti-adhesive constituents. An aqueous acetone-soluble fraction (NDMac) obtained from Sephadex LH-20 inhibited adhesion-linked activities by oral bacteria, including co-aggregation of oral bacteria Fusobacterium nucleatum with Streptococcus sanguinis or Porphyromonas gingivalis, and biofilm formation by Streptococcus mutans. Analysis of NDMac and subsequent subfractions by MALDI-TOF MS and ¹H NMR revealed the presence of A-type proanthocyanidin oligomers (PACs) of 3-6 degrees of polymerization composed of (epi)catechin units, with some (epi)gallocatechin and anthocyanin units also present, as well as quercetin derivatives. Subfractions containing putative xyloglucans in addition to the mixed polyphenols also inhibit biofilm formation by S. mutans (MIC = 125-250 μg mL^-1). These studies suggest that the anti-adhesion activities of cranberry NDM on oral bacteria may arise from a combination of mixed polyphenol and non-polyphenol constituents.

Original language	English
Pages (from-to)	1955-1965
Number of pages	11
Journal	Food and Function
Volume	8
Issue number	5
DOIs	https://doi.org/10.1039/c7fo00109f
State	Published - May 2017

Bibliographical note

Funding Information:
The authors acknowledge NSF-MRI award CHE-1229339, which provided instrumentation support, as well as the assistance of Stephen Eyles and Rinat Abzalimov at the UMass Amherst Mass Spectrometry Facility, and Xin Wang and Liang Xue at UMass Dartmouth. This work was supported by Tel Aviv University and the UMass Cranberry Health Research Center.

Publisher Copyright:
© 2017 The Royal Society of Chemistry.

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Neto, C. C., Penndorf, K. A., Feldman, M., Meron-Sudai, S., Zakay-Rones, Z., Steinberg, D., Fridman, M., Kashman, Y., Ginsburg, I., Ofek, I., & Weiss, E. I. (2017). Characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria. Food and Function, 8(5), 1955-1965. https://doi.org/10.1039/c7fo00109f

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title = "Characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria",

abstract = "An extract prepared from cranberry juice by dialysis known as nondialyzable material (NDM) has been shown previously to possess anti-adhesion activity toward microbial species including oral bacteria, uropathogenic Escherichia coli and Helicobacter pylori. Bioassay-guided fractionation of cranberry NDM was therefore undertaken to identify the anti-adhesive constituents. An aqueous acetone-soluble fraction (NDMac) obtained from Sephadex LH-20 inhibited adhesion-linked activities by oral bacteria, including co-aggregation of oral bacteria Fusobacterium nucleatum with Streptococcus sanguinis or Porphyromonas gingivalis, and biofilm formation by Streptococcus mutans. Analysis of NDMac and subsequent subfractions by MALDI-TOF MS and 1H NMR revealed the presence of A-type proanthocyanidin oligomers (PACs) of 3-6 degrees of polymerization composed of (epi)catechin units, with some (epi)gallocatechin and anthocyanin units also present, as well as quercetin derivatives. Subfractions containing putative xyloglucans in addition to the mixed polyphenols also inhibit biofilm formation by S. mutans (MIC = 125-250 μg mL-1). These studies suggest that the anti-adhesion activities of cranberry NDM on oral bacteria may arise from a combination of mixed polyphenol and non-polyphenol constituents.",

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Neto, CC, Penndorf, KA, Feldman, M, Meron-Sudai, S, Zakay-Rones, Z, Steinberg, D, Fridman, M, Kashman, Y, Ginsburg, I, Ofek, I & Weiss, EI 2017, 'Characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria', Food and Function, vol. 8, no. 5, pp. 1955-1965. https://doi.org/10.1039/c7fo00109f

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AU - Neto, C. C.

AU - Penndorf, K. A.

AU - Feldman, M.

AU - Meron-Sudai, S.

AU - Zakay-Rones, Z.

AU - Steinberg, D.

AU - Fridman, M.

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AU - Ofek, I.

AU - Weiss, E. I.

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Characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria

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