TY - JOUR
T1 - Characterization of novel breast carcinoma-associated BA46-derived peptides in HLA-A2.1/Db-β2m transgenic mice
AU - Carmon, Lior
AU - Bobilev-Priel, Irene
AU - Brenner, Baruch
AU - Bobilev, Dimitry
AU - Paz, Adrian
AU - Bar-Haim, Erez
AU - Tirosh, Boaz
AU - Klein, Tirza
AU - Fridkin, Mati
AU - Lemonnier, Francois
AU - Tzehoval, Esther
AU - Eisenbach, Lea
PY - 2002/8
Y1 - 2002/8
N2 - The human milk fat globule membrane protein BA46 (lactadherin) is highly overexpressed in human breast tumors, making it a potential target for tumor immunotherapy. We have identified BA46-derived peptides that contain the motif recognized by the MHC class I molecule HLA-A2.1 and that are processed and presented by human breast carcinoma cells. In mice lacking normal class I molecules but expressing an HLA-A2.1/Db-β2 microglobulin single chain (HHD mice), three peptides elicited specific CTL activity. Two of these peptides also stimulated cytotoxic activity in peripheral blood lymphocytes from HLA-A2.1-positive breast carcinoma patients. Adoptive transfer of HHD-derived bulk CTLs to nude mice bearing human breast carcinoma transplants reduced tumor growth. These peptides therefore represent naturally processed BA46-derived CTL epitopes that can be used in peptide-based antitumor vaccines.
AB - The human milk fat globule membrane protein BA46 (lactadherin) is highly overexpressed in human breast tumors, making it a potential target for tumor immunotherapy. We have identified BA46-derived peptides that contain the motif recognized by the MHC class I molecule HLA-A2.1 and that are processed and presented by human breast carcinoma cells. In mice lacking normal class I molecules but expressing an HLA-A2.1/Db-β2 microglobulin single chain (HHD mice), three peptides elicited specific CTL activity. Two of these peptides also stimulated cytotoxic activity in peripheral blood lymphocytes from HLA-A2.1-positive breast carcinoma patients. Adoptive transfer of HHD-derived bulk CTLs to nude mice bearing human breast carcinoma transplants reduced tumor growth. These peptides therefore represent naturally processed BA46-derived CTL epitopes that can be used in peptide-based antitumor vaccines.
UR - http://www.scopus.com/inward/record.url?scp=0036679375&partnerID=8YFLogxK
U2 - 10.1172/JCI200214071
DO - 10.1172/JCI200214071
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C2 - 12189239
AN - SCOPUS:0036679375
SN - 0021-9738
VL - 110
SP - 453
EP - 462
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
IS - 4
ER -
