Characterization of permeation pathways appearing in the host membrane of Plasmodium falciparum infected red blood cells

Hagai Ginsburg*, Shirley Kutner, Miriam Krugliak, Z. Ioav Cabantchik

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

169 Scopus citations

Abstract

The host cell membrane of Plasmodium falciparum infected cells becomes permeabilized at the trophozoite stage. A variety of otherwise impermeant substances such as carbohydrates, polyols, amino acids and anions easily gain access to the cytosol of infected cells. Using the isotonic-hemolysis method or uptake of labeled substances, we characterized the new permeation pathways as pores of approximately 0.7 nm equivalent radius. The pores bear a positively charged character which facilitates movement of small anions and excludes cations, so that the ionic composition and osmotic properties of infected cells are not drastically altered. Substances of a molecular size similar to that of disaccharides are fully excluded. Substances of limiting size might be accommodated in the pore, provided they bear a side group of hydrophobic character. The new permeation pathways may provide a vital route for acquisition or release of essential nutrients or catabolites.

Original languageEnglish
Pages (from-to)313-322
Number of pages10
JournalMolecular and Biochemical Parasitology
Volume14
Issue number3
DOIs
StatePublished - Mar 1985

Keywords

  • Hemolysis
  • Human erythrocytes
  • Membrane permeability
  • Plasmodium falciparum

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