TY - JOUR
T1 - Characterization of permeation pathways appearing in the host membrane of Plasmodium falciparum infected red blood cells
AU - Ginsburg, Hagai
AU - Kutner, Shirley
AU - Krugliak, Miriam
AU - Ioav Cabantchik, Z.
PY - 1985/3
Y1 - 1985/3
N2 - The host cell membrane of Plasmodium falciparum infected cells becomes permeabilized at the trophozoite stage. A variety of otherwise impermeant substances such as carbohydrates, polyols, amino acids and anions easily gain access to the cytosol of infected cells. Using the isotonic-hemolysis method or uptake of labeled substances, we characterized the new permeation pathways as pores of approximately 0.7 nm equivalent radius. The pores bear a positively charged character which facilitates movement of small anions and excludes cations, so that the ionic composition and osmotic properties of infected cells are not drastically altered. Substances of a molecular size similar to that of disaccharides are fully excluded. Substances of limiting size might be accommodated in the pore, provided they bear a side group of hydrophobic character. The new permeation pathways may provide a vital route for acquisition or release of essential nutrients or catabolites.
AB - The host cell membrane of Plasmodium falciparum infected cells becomes permeabilized at the trophozoite stage. A variety of otherwise impermeant substances such as carbohydrates, polyols, amino acids and anions easily gain access to the cytosol of infected cells. Using the isotonic-hemolysis method or uptake of labeled substances, we characterized the new permeation pathways as pores of approximately 0.7 nm equivalent radius. The pores bear a positively charged character which facilitates movement of small anions and excludes cations, so that the ionic composition and osmotic properties of infected cells are not drastically altered. Substances of a molecular size similar to that of disaccharides are fully excluded. Substances of limiting size might be accommodated in the pore, provided they bear a side group of hydrophobic character. The new permeation pathways may provide a vital route for acquisition or release of essential nutrients or catabolites.
KW - Hemolysis
KW - Human erythrocytes
KW - Membrane permeability
KW - Plasmodium falciparum
UR - http://www.scopus.com/inward/record.url?scp=0021992698&partnerID=8YFLogxK
U2 - 10.1016/0166-6851(85)90059-3
DO - 10.1016/0166-6851(85)90059-3
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C2 - 3887158
AN - SCOPUS:0021992698
SN - 0166-6851
VL - 14
SP - 313
EP - 322
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 3
ER -