Characterization of polar membrane lipids of the extremely halophilic bacterium Salinibacter ruber and possible role of cardiolipin

Veronica M.T. Lattanzio, Maristella Baronio, Aharon Oren, Nicholas J. Russell, Angela Corcelli*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The lipid composition of the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) was investigated by thin layer chromatography, gas chromatography, high performance liquid chromatography and electrospray ionization-mass spectrometry. Polar lipids represent about 80% of the total lipid extract. The main polar lipids are a sulfonic acid analogue of ceramide (or capnine analogue), phosphatidylcholine, phosphatidylserine, dimethylphosphatidylethanolamine, phosphatidylglycerol, cardiolipin or bisphosphatidylglycerol, and a glycolipid. The major acyl chains in the phospholipids are C16:1 Δ9cis and C18:1 Δ11cis, while the sulfonolipid contains an amide-bound iso C15:0 fatty acid. On changing the salinity of the culture medium, no significant differences were found in the lipid profile or the unsaturation of the lipid fatty acyl chains. The structure of the cardiolipin, which represents 20% of polar lipids, has been elucidated by gas chromatography and electrospray ionization mass spectrometry analysis.

Original languageAmerican English
Pages (from-to)25-31
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1791
Issue number1
DOIs
StatePublished - Jan 2009

Bibliographical note

Funding Information:
This work was supported by the Ministero Italiano dell'Università e della Ricerca (Fondi Ateneo), the Ministero Italiano della Difesa (Contract no. 1999/13.12.2005) and the Istituto per I Processi Chimico-Fisici del consiglio Nazionale delle Ricerche, Bari, Italy (CNR-IPCF). We thank G. Mascolo (CNR-IRSA, Bari) for ESI-MS analyses.

Funding Information:
Aharon Oren's studies on Salinibacter are supported by the Israel Science Foundation (grant no. 617/07).

Keywords

  • Bacteroidetes
  • Bisphosphatidylglycerol
  • Cardiolipin
  • Halophilic
  • Salinibacter ruber
  • Sulfonolipids

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