Abstract
The lipid composition of the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) was investigated by thin layer chromatography, gas chromatography, high performance liquid chromatography and electrospray ionization-mass spectrometry. Polar lipids represent about 80% of the total lipid extract. The main polar lipids are a sulfonic acid analogue of ceramide (or capnine analogue), phosphatidylcholine, phosphatidylserine, dimethylphosphatidylethanolamine, phosphatidylglycerol, cardiolipin or bisphosphatidylglycerol, and a glycolipid. The major acyl chains in the phospholipids are C16:1 Δ9cis and C18:1 Δ11cis, while the sulfonolipid contains an amide-bound iso C15:0 fatty acid. On changing the salinity of the culture medium, no significant differences were found in the lipid profile or the unsaturation of the lipid fatty acyl chains. The structure of the cardiolipin, which represents 20% of polar lipids, has been elucidated by gas chromatography and electrospray ionization mass spectrometry analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 25-31 |
| Number of pages | 7 |
| Journal | Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids |
| Volume | 1791 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2009 |
Bibliographical note
Funding Information:This work was supported by the Ministero Italiano dell'Università e della Ricerca (Fondi Ateneo), the Ministero Italiano della Difesa (Contract no. 1999/13.12.2005) and the Istituto per I Processi Chimico-Fisici del consiglio Nazionale delle Ricerche, Bari, Italy (CNR-IPCF). We thank G. Mascolo (CNR-IRSA, Bari) for ESI-MS analyses.
Funding Information:
Aharon Oren's studies on Salinibacter are supported by the Israel Science Foundation (grant no. 617/07).
Keywords
- Bacteroidetes
- Bisphosphatidylglycerol
- Cardiolipin
- Halophilic
- Salinibacter ruber
- Sulfonolipids