Characterization of soman-binding antibodies raised against soman analogs

Michael Glikson, Rina Arad-Yellin, Michal Ghozi, Lily Raveh, Bernard S. Green*, Zelig Eshhar

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The production of antibodies against the highly toxic organophosphorus compound soman (GD) has been undertaken. Monoclonal antibodies were raised against two structural analogs of soman which served as haptens for immunization. In these soman analogs the chemically active PF bond of the soman molecule was substituted by a POH group (which is ionized to PO- under physiological conditions) or a PH bond, creating compounds which we have named GDOH and GDH, respectively. These soman analogs were linked to carrier proteins through a short linker extending from the pinacolyl group. Monoclonal antibodies were selected according to their ability to bind to the immunizing hapten, and their specificities were determined by competitive inhibition assays. Out of total of 103 anti-GDOH antibodies 22 bound soman, whereas no binding was achieved with 62 anti-GDH antibodies. The two groups of monoclonal antibodies differed also in their structural specificity as demonstrated by different reactivities against a variety of soman analogs and substituted derivatives. These studies indicate that in order to achieve further improvement in anti-soman reactivity with protective potential, other groups (which resemble the OH group) have to be substituted for the F atom in the soman molecule.

Original languageEnglish
Pages (from-to)903-910
Number of pages8
JournalMolecular Immunology
Volume29
Issue number7-8
DOIs
StatePublished - 1992

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