TY - JOUR
T1 - Characterization of soman-binding antibodies raised against soman analogs
AU - Glikson, Michael
AU - Arad-Yellin, Rina
AU - Ghozi, Michal
AU - Raveh, Lily
AU - Green, Bernard S.
AU - Eshhar, Zelig
PY - 1992
Y1 - 1992
N2 - The production of antibodies against the highly toxic organophosphorus compound soman (GD) has been undertaken. Monoclonal antibodies were raised against two structural analogs of soman which served as haptens for immunization. In these soman analogs the chemically active PF bond of the soman molecule was substituted by a POH group (which is ionized to PO- under physiological conditions) or a PH bond, creating compounds which we have named GDOH and GDH, respectively. These soman analogs were linked to carrier proteins through a short linker extending from the pinacolyl group. Monoclonal antibodies were selected according to their ability to bind to the immunizing hapten, and their specificities were determined by competitive inhibition assays. Out of total of 103 anti-GDOH antibodies 22 bound soman, whereas no binding was achieved with 62 anti-GDH antibodies. The two groups of monoclonal antibodies differed also in their structural specificity as demonstrated by different reactivities against a variety of soman analogs and substituted derivatives. These studies indicate that in order to achieve further improvement in anti-soman reactivity with protective potential, other groups (which resemble the OH group) have to be substituted for the F atom in the soman molecule.
AB - The production of antibodies against the highly toxic organophosphorus compound soman (GD) has been undertaken. Monoclonal antibodies were raised against two structural analogs of soman which served as haptens for immunization. In these soman analogs the chemically active PF bond of the soman molecule was substituted by a POH group (which is ionized to PO- under physiological conditions) or a PH bond, creating compounds which we have named GDOH and GDH, respectively. These soman analogs were linked to carrier proteins through a short linker extending from the pinacolyl group. Monoclonal antibodies were selected according to their ability to bind to the immunizing hapten, and their specificities were determined by competitive inhibition assays. Out of total of 103 anti-GDOH antibodies 22 bound soman, whereas no binding was achieved with 62 anti-GDH antibodies. The two groups of monoclonal antibodies differed also in their structural specificity as demonstrated by different reactivities against a variety of soman analogs and substituted derivatives. These studies indicate that in order to achieve further improvement in anti-soman reactivity with protective potential, other groups (which resemble the OH group) have to be substituted for the F atom in the soman molecule.
UR - http://www.scopus.com/inward/record.url?scp=0026649593&partnerID=8YFLogxK
U2 - 10.1016/0161-5890(92)90128-K
DO - 10.1016/0161-5890(92)90128-K
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C2 - 1635561
AN - SCOPUS:0026649593
SN - 0161-5890
VL - 29
SP - 903
EP - 910
JO - Molecular Immunology
JF - Molecular Immunology
IS - 7-8
ER -