Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato

Evyatar Steiner, Alon Israeli, Rupali Gupta, Ido Shwartz, Ido Nir, Meirav Leibman-Markus, Lior Tal, Mika Farber, Ziva Amsalem, Naomi Ori, Bruno Müller, Maya Bar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Background: Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development. Results: Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato. Conclusions: The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers.

Original languageAmerican English
Article number152
JournalPlant Methods
Volume16
Issue number1
DOIs
StatePublished - Dec 2020

Bibliographical note

Publisher Copyright:
© 2020, The Author(s).

Keywords

  • Arabidopsis
  • Cytokinin
  • Imaging
  • TCSv2
  • Tomato
  • Two-component sensor (TCS)

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