TY - JOUR
T1 - Characterization of the inhibitory dopamine receptor from the pituitary of tilapia
AU - Berta, Levavi Sivan
AU - Ayelet, Avitan
AU - Tamir, Kanias
N1 - Funding Information:
This research was supported by the Israel Science Foundation No. 775/01.
PY - 2003
Y1 - 2003
N2 - The study was aimed to characterize the inhibitory dopamine (DA) receptor in the pituitary of tilapia. DA D2 and D3 agonists inhibited GnRH-stimulated LH release from tilapia pituitary cells more efficiently than D4 agonists. However, no significant differences were observed between the efficiencies of D2 and D3 drugs. In order to elucidate the signal transduction of the tilapia DA receptor we performed transfection of COS-7 cells with tilapia D2 DA receptor that resulted in the inhibition of forskolin-stimulated cAMP accumulation. Moreover, the tilapia D2 dopamine receptor inhibits adenylate cyclase activity via coupling to pertussis toxin-sensitive G proteins of the Gi/Go family. Our results also show that estradiol up-regulates taDA-R mRNA.
AB - The study was aimed to characterize the inhibitory dopamine (DA) receptor in the pituitary of tilapia. DA D2 and D3 agonists inhibited GnRH-stimulated LH release from tilapia pituitary cells more efficiently than D4 agonists. However, no significant differences were observed between the efficiencies of D2 and D3 drugs. In order to elucidate the signal transduction of the tilapia DA receptor we performed transfection of COS-7 cells with tilapia D2 DA receptor that resulted in the inhibition of forskolin-stimulated cAMP accumulation. Moreover, the tilapia D2 dopamine receptor inhibits adenylate cyclase activity via coupling to pertussis toxin-sensitive G proteins of the Gi/Go family. Our results also show that estradiol up-regulates taDA-R mRNA.
UR - http://www.scopus.com/inward/record.url?scp=8844267584&partnerID=8YFLogxK
U2 - 10.1023/B:FISH.0000030479.47055.24
DO - 10.1023/B:FISH.0000030479.47055.24
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AN - SCOPUS:8844267584
SN - 0920-1742
VL - 28
SP - 73
EP - 75
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
IS - 1-4
ER -
