Characterization of the inhibitory dopamine receptor from the pituitary of tilapia

Levavi Sivan Berta; Avitan Ayelet; Kanias Tamir

doi:10.1023/B:FISH.0000030479.47055.24

Characterization of the inhibitory dopamine receptor from the pituitary of tilapia

Levavi Sivan Berta^*
, Avitan Ayelet
, Kanias Tamir

^*Corresponding author for this work

Department of Animal Sciences

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The study was aimed to characterize the inhibitory dopamine (DA) receptor in the pituitary of tilapia. DA D₂ and D₃ agonists inhibited GnRH-stimulated LH release from tilapia pituitary cells more efficiently than D₄ agonists. However, no significant differences were observed between the efficiencies of D₂ and D₃ drugs. In order to elucidate the signal transduction of the tilapia DA receptor we performed transfection of COS-7 cells with tilapia D₂ DA receptor that resulted in the inhibition of forskolin-stimulated cAMP accumulation. Moreover, the tilapia D₂ dopamine receptor inhibits adenylate cyclase activity via coupling to pertussis toxin-sensitive G proteins of the Gi/Go family. Our results also show that estradiol up-regulates taDA-R mRNA.

Original language	English
Pages (from-to)	73-75
Number of pages	3
Journal	Fish Physiology and Biochemistry
Volume	28
Issue number	1-4
DOIs	https://doi.org/10.1023/B:FISH.0000030479.47055.24
State	Published - 2003

Bibliographical note

Funding Information:
This research was supported by the Israel Science Foundation No. 775/01.

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Cite this

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title = "Characterization of the inhibitory dopamine receptor from the pituitary of tilapia",

abstract = "The study was aimed to characterize the inhibitory dopamine (DA) receptor in the pituitary of tilapia. DA D2 and D3 agonists inhibited GnRH-stimulated LH release from tilapia pituitary cells more efficiently than D4 agonists. However, no significant differences were observed between the efficiencies of D2 and D3 drugs. In order to elucidate the signal transduction of the tilapia DA receptor we performed transfection of COS-7 cells with tilapia D2 DA receptor that resulted in the inhibition of forskolin-stimulated cAMP accumulation. Moreover, the tilapia D2 dopamine receptor inhibits adenylate cyclase activity via coupling to pertussis toxin-sensitive G proteins of the Gi/Go family. Our results also show that estradiol up-regulates taDA-R mRNA.",

author = "Berta, \{Levavi Sivan\} and Avitan Ayelet and Kanias Tamir",

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T1 - Characterization of the inhibitory dopamine receptor from the pituitary of tilapia

AU - Berta, Levavi Sivan

AU - Ayelet, Avitan

AU - Tamir, Kanias

N1 - Funding Information: This research was supported by the Israel Science Foundation No. 775/01.

PY - 2003

Y1 - 2003

N2 - The study was aimed to characterize the inhibitory dopamine (DA) receptor in the pituitary of tilapia. DA D2 and D3 agonists inhibited GnRH-stimulated LH release from tilapia pituitary cells more efficiently than D4 agonists. However, no significant differences were observed between the efficiencies of D2 and D3 drugs. In order to elucidate the signal transduction of the tilapia DA receptor we performed transfection of COS-7 cells with tilapia D2 DA receptor that resulted in the inhibition of forskolin-stimulated cAMP accumulation. Moreover, the tilapia D2 dopamine receptor inhibits adenylate cyclase activity via coupling to pertussis toxin-sensitive G proteins of the Gi/Go family. Our results also show that estradiol up-regulates taDA-R mRNA.

AB - The study was aimed to characterize the inhibitory dopamine (DA) receptor in the pituitary of tilapia. DA D2 and D3 agonists inhibited GnRH-stimulated LH release from tilapia pituitary cells more efficiently than D4 agonists. However, no significant differences were observed between the efficiencies of D2 and D3 drugs. In order to elucidate the signal transduction of the tilapia DA receptor we performed transfection of COS-7 cells with tilapia D2 DA receptor that resulted in the inhibition of forskolin-stimulated cAMP accumulation. Moreover, the tilapia D2 dopamine receptor inhibits adenylate cyclase activity via coupling to pertussis toxin-sensitive G proteins of the Gi/Go family. Our results also show that estradiol up-regulates taDA-R mRNA.

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JO - Fish Physiology and Biochemistry

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ER -

Characterization of the inhibitory dopamine receptor from the pituitary of tilapia

Abstract

Bibliographical note

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