Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization

Joseph Aizen; Noga Kowalsman; Masha Y. Niv; Berta Levavi-Sivan

doi:10.1016/j.ygcen.2014.05.028

Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization

Joseph Aizen, Noga Kowalsman, Masha Y. Niv, Berta Levavi-Sivan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia.

Original language	American English
Pages (from-to)	28-33
Number of pages	6
Journal	General and Comparative Endocrinology
Volume	207
DOIs	https://doi.org/10.1016/j.ygcen.2014.05.028
State	Published - 1 Oct 2014

Bibliographical note

Funding Information:
This work was presented in a workshop that was supported by BARD, the United States – Israel Binational Agricultural Research and Development Fund, Workshop No. W-94-13. We thank Dr. Talia Yarnitzki for help with the modeling, and Prof. D. Kime for the anti-11-ketotestosterone.

Publisher Copyright:
© 2014 Elsevier Inc.

Keywords

11-ketotestosterone
FSH
GnRH
Gonadotropin receptor
LH

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10.1016/j.ygcen.2014.05.028

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title = "Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization",

abstract = "In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia.",

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author = "Joseph Aizen and Noga Kowalsman and Niv, {Masha Y.} and Berta Levavi-Sivan",

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N2 - In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia.

AB - In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia.

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Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization

Abstract

Bibliographical note

Keywords

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