Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization

Joseph Aizen, Noga Kowalsman, Masha Y. Niv, Berta Levavi-Sivan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia.

Original languageAmerican English
Pages (from-to)28-33
Number of pages6
JournalGeneral and Comparative Endocrinology
StatePublished - 1 Oct 2014

Bibliographical note

Publisher Copyright:
© 2014 Elsevier Inc.


  • 11-ketotestosterone
  • FSH
  • GnRH
  • Gonadotropin receptor
  • LH


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