Characterization of tyrosine protein kinase associated with subcellular components of human colonic epithelium

B. Schwartz, E. Cagnano, S. Braun, S. A. Lamprecht*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Tyrosine protein kinase (TPK) co-isolated with subcellular components derived from human colonic epithelium. The highest TPK activity, measured in the Triton X-100-insoluble cytoskeletal pellet, was directly related to the degree of malignancy of colonic tissue. TPK activity was assayed by measuring the incorporation of [γ-32P] from [γ32]ATP into the synthetic polymer [Glu80Tyr20](n) substrate. Lineweaver-Burk plots yielded an apparent Km of 167 μg/ml for [Glu80Tyr20](n) and of 19μM for ATP: V(max) for the phosphate donor was 0.9 nmol/min/mg protein. TPK activity was markedly stimulated by the metal ions Mg2+ and Mn2+ and significantly suppressed by tyrphostins, potent specific TPK inhibitors, shown to interfere with TPK-dependent growth processes. This is the first report to present evidence for TPK activity associated with cytoskeleton-enriched subcellular preparations harvested from human colonic epithelium.

Original languageEnglish
Pages (from-to)1747-1753
Number of pages7
JournalAnticancer Research
Volume10
Issue number6
StatePublished - 1990
Externally publishedYes

Keywords

  • colon
  • cytoskeleton
  • neoplasia
  • tyrosine protein kinase

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