Chemical genetic analysis of Apg1 reveals a non-kinase role in the induction of autophagy

Hagai Abeliovich, Chao Zhang, William A. Dunn, Kevan M. Shokat, Daniel J. Klionsky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

137 Scopus citations


Macroautophagy is a catabolic membrane trafficking phenomenon that is observed in all eukaryotic cells in response to various stimuli, such as nitrogen starvation and challenge with specific hormones. In the yeast Saccharomyces cerevisiae, the induction of autophagy involves a direct signal transduction mechanism that affects membrane dynamics. In this system, the induction process modifies a constitutive trafficking pathway called the cytoplasm-to-vacuole targeting (Cvt) pathway, which transports the vacuolar hydrolase aminopeptidase I, from the formation of small Cvt vesicles to the formation of autophagosomes. Apg1 is one of the proteins required for the direct signal transduction cascade that modifies membrane dynamics. Although Apg1 is required for both the Cvt pathway and autophagy, we find that Apg1 kinase activity is required only for Cvt trafficking of aminopeptidase I but not for import via autophagy. In addition, the data support a novel role for Apg1 in nucleation of autophagosomes that is distinct from its catalytic kinase activity and imply a qualitative difference in the mechanism of autophagosome and Cvt vesicle formation.

Original languageAmerican English
Pages (from-to)477-490
Number of pages14
JournalMolecular Biology of the Cell
Issue number2
StatePublished - 1 Feb 2003
Externally publishedYes


Dive into the research topics of 'Chemical genetic analysis of Apg1 reveals a non-kinase role in the induction of autophagy'. Together they form a unique fingerprint.

Cite this